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Shh (C25II) Protein, Mouse

Sonic Hedgehog,C24II.,HHG1

价格 ￥810.00 （ 1-2周）
货号 UA040100
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Mouse

  • 抗原名称

    Shh

  • 分子别名

    Sonic Hedgehog,C24II.,HHG1

  • Accession

    Q62226

  • 表达序列

    Cys25-Gly198 (Cys25Ile-Ile)

  • 表达宿主

    E.coli

  • 分子量

    20kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM Tris, 150mM NaCl, pH8.0.

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Marigo V, et al. (1995) Cloning, expression, and chromosomal location of SHH and IHH: two human homologues of the Drosophila segment polarity gene hedgehog. Genomics. 1;28(1):44-51.
    2.Gomes DC, et al. (2014) Sonic hedgehog signaling is active in human adrenal cortex development and deregulated in adrenocortical tumors. J Clin Endocrinol Metab. 99(7):E1209-16.

背景介绍

  • Sonic Hedgehog (SHH) is a secreted factor which expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. SHH binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of SHH, patched receptor represses the constitutive signaling activity of smoothened. SHH also regulates another factor, the gli oncogene. It regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. SHH-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at itsThe Shh-N protein is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its N-terminal cysteine and modified by cholesterol addition at its C-terminus. These modifications contribute to the membrane tethering of the Shh protein as well as its assembly into various sized multimers.

  • 生物活性

    • Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The EC50 for this effect is less than 0.25µg/ml.

  • 电泳

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

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