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物种
Rat分子别名
FGF18,fibroblast growth factor 18,zFGF5Accession
O88182表达序列
Glu28-Arg199表达宿主
E.coli分子量
20kDa纯度
>95% by SDS-PAGE & RP-HPLC内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20 mM Tris, 600 mM NaCl, pH 8.5溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1、Davidson D. et al. (2005) Fibroblast growth factor (FGF) 18 signals through FGF receptor 3 to promote chondrogenesis. J Biol Chem. 280(21): 20509-15.
2、Liu Z. et al. (2007) FGF18 is required for early chondrocyte proliferation, hypertrophy and vascular invasion of the growth plate. Dev Biol. 302(1): 80-91.
Fibroblast Growth Factor 18 (FGF-18) plays an important role in skeletal development and bone homeostasis. Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18. It is expressed in embryonic somites and the neural fold, adult lung, cerebellar and hippocampal neurons, hair follicle root sheath cells, and osteogenic mesenchymal cells. FGF-18 is a heparin-binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-18 is required for normal skeletal development. Recombinant Human FGF-18 derived from E.coli is a 20 kDa protein consisting of 173 amino acid residues, resulting from C-terminal truncation of the full length protein.
生物活性
- Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The EC50 for this effect is less than 10ng/ml.
电泳
- 2μg (R: reducing condition, N: non-reducing condition).
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