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物种
Human, Human抗原名称
IL-8分子别名
Interleukin-8, IL8, CXCL8, NAP-1, NAF;Accession
P10145表达序列
Ser28-Ser99
表达宿主
E.coli分子量
9kDa (Reducing)
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
Measured by human U-87 MG cells. The EC50 for this effect is less than 10ng/ml.标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Van Damme J, Rampart M, Conings R, et al. 1990. Eur J Immunol. 20:2113-8.
IL-8(Interleukin-8) is one of the first discovered chemokines and belongs to the CXCL family, in which the first two conserved cysteines are separated by one residue. In vivo, IL-8 exists in two forms: a 77-amino acid protein produced by endothelial cells, and the more active 72- amino acid protein produced by monocytes. IL-8 is often associated with inflammation, it has been cited as a proinflammatory mediator in gingivitis and psoriasis. The functions of IL-8 are to induce rapid changes in cell morphology, activate integrins, and release the granule contents of neutrophils. Thus, IL-8 can enhance the antimicrobial actions of defense cells. It is secreted by monocytes, macrophages and endothelial cells. IL-8 signals through CXCR1 and CXCR2 to chemoattract neutrophils, basophils, and T cells. IL-8 is also a potent promoter of angiogenesis.
电泳
- 2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized IL-8 (28-99) Protein, Human (Cat. No.UA040103) at 1 µg/mL (100 µL/well) can bind Adakitug with a linear range of 0.37–0.74 ng/mL
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