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物种
Mouse分子别名
FGF-1,Acidic fibroblast growth factor(aFGF),Heparin-binding growth factor 1(HBGF-1)Accession
P61148表达序列
Phe16-Asp155
表达宿主
E.coli分子量
17-18kDa
纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg活性
< 0.5ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM PB, 150mM NaCl, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1、Zakrzewska M. et al. (2008) FGF-1: from biology through engineering to potential medical applications. Crit Rev Clin Lab Sci. 45(1): 91-135.
2、Pye D A. et al. (2000) Regulation of FGF-1 mitogenic activity by heparan sulfate oligosaccharides is dependent on specific structural features: differential requirements for the modulation of FGF-1 and FGF-2. Glycobiology. 10(11): 1183-1192.
Fibroblast growth factor 1 (FGF-1), known as acidic fibroblast growth factor (FGF-Acidic), is one of the best characterized members of the FGF superfamily. FGF-1 is a powerful mitogen exhibiting strong action on numerous different cell types. It plays a role in various stages of development and morphogenesis, as well as in angiogenesis and wound healing processes. FGF-1 is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF-1 with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes.
生物活性
- Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The EC50 for this effect is less than 0.5ng/ml.
电泳
- 1μg (R: reducing condition, N: non-reducing condition).
SPR
Anti-His antibody Immobilized on CM5 Chip captured FGF-R2 (IIIb) His Tag Protein, Human(Cat. No. UA010145), can bind FGF-1 Protein, Mouse(Cat. No. UA040089) with an affinity constant of 2.22 nM as determined in SPR assay.
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