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物种
Human分子别名
FGF9,GAF,HBFG-9,MGC119914,MGC119915,SYNS3Accession
P31371表达序列
Met1-Ser208表达宿主
E.coli分子量
25kDa
纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg活性
<1ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 500mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1、Schmahl J. et al. (2004) Fgf9 induces proliferation and nuclear localization of FGFR2 in Sertoli precursors during male sex determination. Development. 131(15): 3627-36.
2、Garcès A. et al. (2000) FGF9: a motoneuron survival factor expressed by medial thoracic and sacral motoneurons. J Neurosci Res. 60(1): 1-9.
FGF9(fibroblast growth factor 9, FGF9) is one of the fibroblast growth factor (FGF) family members. It is widely distributed in various tissues of human body and involved in bone development, angiogenesis, embryonic development, damage repair, cell apoptosis, nerve regeneration, tumor growth and other physiological and pathological processes, and can effectively promote mitosis and cell growth. Current evidence suggests that FGF9 may play a prominent role not only in bone development and growth of cartilage into bone formation and generation, but also has a significant impact on aspects of ovarian cancer, nerve regeneration, gonadal differentiation, there remains much to be discovered and investigated on the functions and mechanisms of FGF18.
生物活性
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells, the EC50 for this effect is less than 1ng/ml.
电泳
- 5μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized FGF-9 Protein, Human (Cat. No. UA040087) at 5.0μg/mL (100μL/well) can bind Human FGF R2 (IIIb) protein, Fc Tag with EC50 of 13.99-18.37ng/mL.
SPR
CM5 Chip captured FGF-9, Human(Cat. No. UA040087), can bind FGF-R2 (IIIb) His Tag, Human(Cat. No. UA010145) with an affinity constant of 0.45μM as determined in SPR assay.
CM5 Chip captured FGF-9, Human(Cat. No. UA040087), can bind FGF-R4/CD334 His Tag, Human (Cat. No. UA020009) with an affinity constant of 0.28μM as determined in SPR assay.
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