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物种
Human抗原名称
FGF-19分子别名
FGF-19Accession
O95750表达序列
Leu25-Lys216
表达宿主
E.coli分子量
24kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
<100ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 200mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Rabia Bag Soytas, Veysel Suzan, Pinar Arman. Association of FGF-19 and FGF-21 levels with primary sarcopenia. Geriatr Gerontol Int. 2021 Oct;21(10):959-962.Epub 2021 Aug 17.
2. Nobuyuki Itoh 1, David M Ornitz. Functional evolutionary history of the mouse Fgf gene family. Dev Dyn. 2008 Jan;237(1):18-27.
Fibroblast Growth Factors (FGFs) are polypeptides with diverse activities in development and physiology. The mammalian Fgf family can be divided into the intracellular Fgf11/12/13/14 subfamily (iFGFs), the hormone-like Fgf15/21/23 subfamily (hFGFs), and the canonical Fgf subfamilies, including Fgf1/2/5, Fgf3/4/6, Fgf7/10/22, Fgf8/17/18, and Fgf9/16/20. FGF-19 is secreted from ileal enterocytes similar to a hormone. Endocrine derived hormone FGF-19 has recently emerged as a potential target for the treatment of metabolic diseases. In recognition of the skeletal muscle as an important metabolic organ, it is considered that FGF-19 may also have a role in muscle metabolism and may be a new factor with promising value in the treatment of sarcopenia.
生物活性
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells.The EC50 for this effect is less than 100ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
ELISA
Immobilized FGF-19 Protein, Human (Cat. No. UA040086) at 10.0μg/mL (100μL/well) can bind Klotho β Protein, Human (Cat. No. UA040050) with EC50 of 0.35-0.57 μg/mL.
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