TNF-α Protein, Mouse

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货号： UA040083

大包装询价

产品介绍引用文献(0) 评论(0)

产品规格

物种

Mouse
分子别名

TNF-α, Cachectin, Tumor Necrosis Factor-Alpha, TNFSF1A
Accession

P06804
表达序列

Leu80-Leu235, with N-terminal 6*His MHHHHHHLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL
表达宿主

E.coli
分子量

18.2kDa
纯度

>95% by SDS-PAGE
内毒素含量

<1EU/μg
标记

Unconjugated
标签

His Tag
性状

Lyophilized Powder
缓冲体系

20mM Tris, 150mM NaCl, 2mM TCEP, pH8.0
溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用

1、Pennica D. et al. (1984) Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature. 312(5996): 724-729.
2、Nedospasov S A. et al. (1986) Tandem arrangement of genes coding for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) in the human genome. Cold Spring Harb Symp Quant Biol. 51(1): 611-624.

背景介绍

Tumor necrosis factor α (TNF alpha) is an effective pro-inflammatory cytokine, which can kill and inhibit tumor cells. It is mainly produced by activated macrophages and can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neuronal tissues. The members of TNF alpha family exert their cellular effect through two distinct surface receptors of the TNF receptor family, TNFR1and TNFR2. The pleiotropic biological effects of TNF can be attributed to its ability to simultaneously activate multiple signaling pathways in cells. TNF-induced NF-κB activation promotes the growth, invasion and metastasis of cancer cells.

生物活性
- Measured in a cell proliferation assay using L-929 mouse fibrosarcoma cells, the EC50 for this effect is less than 80 pg/ml.
电泳(SDS-PAGE)
- 1μg (R: reducing condition, N: non-reducing condition).
SPR
- Protein A Chip captured TNFR-1/CD120a Fc Chimera, Human (Cat. No. UA040030), can bind TNF-α His Tag, Mouse (Cat. No. UA040083) with an affinity constant of 0.069nM as determined in SPR assay.