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物种
Human抗原名称
VEGF-121分子别名
Vascular Endothelial Growth Factor-A, VEGFAAccession
P15692-9表达序列
Ala27-Arg147
表达宿主
E.coli分子量
14kDa
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized powder缓冲体系
20mM PB, 150mM NaCl, pH 7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1.FASEB J. 2001 Jul;15(9):1667-9.
2.Cancer Gene Ther. 2016 May;23(5):125-32. Epub 2016 Apr 1.
VEGF-121, an abundant splicing isoform of vascular endothelial growth factor (VEGF), exists as a disulfide-linked homodimer. It induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Contrast to other isoforms, VEGF-121 has very little affinity for heparin-sulfate proteoglycans, without recruiting Neuropilin1-expressing monocytes (NEMs), conferring to VEGF-121 the ability to diffuse freely, and increase tumor growth rate in vivo.
生物活性JSON
Measured in a cell proliferation assay using HUVEC cells，the EC50 for this effect is less than 2ng/ml.
Immobilized VEGF-121, Human (Cat. No. UA040082) at 0.5μg/mL (100μL/well) can bind Bevacizumab (starter-bio, Cat. No. S0B0437) with EC50 of 1.20-1.54 ng/mL.
电泳JSON
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized VEGF-121, Human (Cat. No. UA040082) at 2.0μg/mL (100μL/well) can bind VEGF-R2/KDR Fc Chimera, Human (Cat. No. UA010144) with EC50 of 8.25-11.55 ng/mL.
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