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物种
Human抗原名称
FGF-21分子别名
Fibroblast growth factor 21Accession
Q9NSA1表达序列
His29-Ser209
表达宿主
E.coli分子量
24kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
1 μg/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 150mM NaCl, pH 8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. J Clin Invest. 2005 Jun;115(6):1627-35.Epub 2005 May 2.
2. Exp Gerontol. 2020 Oct 1;139:111022.
Fibroblast Growth Factor 21(FGF-21) is a member of the fibroblast growth factor (FGF) family and it is involved in the modulation of cell proliferation, growth, and differentiation as well as cell metabolism. This protein is a secreted endocrine factor that functions as a major metabolic regulator, and stimulates the uptake of glucose in adipose tissue. FGF21 binds to FGFR1c complexed with β-Klotho expressed in adipocytes to activate the canonical FGF signaling pathway (ERK1/2) and the AMPK/PGC1α pathway. Therefore, FGF-21 is being pursued as a therapeutic target for diabetes and obesity.
生物活性
Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The EC50 this effect is less than 0.5 μg/ml in the presence of 1.25 µg/ml mouse Klotho and 10 µg/ml heparin.
电泳
1μg (R: reducing condition, N: non-reducing condition).
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