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物种
Mouse分子别名
FGF-21,Fibroblast growth factor 21Accession
Q9JJN1表达序列
Ala29-Ser210
表达宿主
E.coli分子量
20kDa
纯度
>95% by SDS-PAGE & RP-HPLC内毒素含量
<1EU/μg活性
EC50 < 0.8μg/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM PB, 150mM NaCl, pH 7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1、Kharitonenkov A J. et al. (2005) FGF-21 as a novel metabolic regulator. J Clin Invest. 115(6): 1627-1635.
2、Guo A. et al. (2020) Sarcopenic obesity: Myokines as potential diagnostic biomarkers and therapeutic targets? Exp Gerontol. 139: 111022.
3、Gomarasca M. et al. (2020) Myokines: The endocrine coupling of skeletal muscle and bone. Adv Clin Chem. 94: 155-218.
Fibroblast Growth Factor 21(FGF-21) is a member of the fibroblast growth factor (FGF) family and it is involved in the modulation of cell proliferation, growth, and differentiation as well as cell metabolism. This protein is a secreted endocrine factor that functions as a major metabolic regulator, and stimulates the uptake of glucose in adipose tissue. FGF21 binds to FGFR1c complexed with β-Klotho expressed in adipocytes to activate the canonical FGF signaling pathway (ERK1/2) and the AMPK/PGC1α pathway. Therefore, FGF-21 is being pursued as a therapeutic target for diabetes and obesity.
电泳
- 2μg (R: reducing condition, N: non-reducing condition).
SPR
Anti-His antibody Immobilized on CM5 Chip captured Klotho β Protein, Mouse (Cat. No. UA040364), can bind FGF-21 Protein, , Mouse (Cat. No. UA040070) with an affinity constant of 33.01nM as determined in SPR assay.
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