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物种
Mouse分子别名
LIF,Leukemia Inhibitory Factor,HILDA,MLPLI,CDF,DIAAccession
P09056表达序列
Ser24-Phe203
表达宿主
E.coli分子量
20kDa
纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 200mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1、Ryffel B. et al. (1993) Pathology induced by leukemia inhibitory factor. International Review of Experimental Pathology. 34: 69-72.
The leukemia inhibitory factor (LIF) belongs to the interleukin-6 (IL-6) cytokine family, which is involved in various biological processes including inflammation, immune responses, and embryonic development. Leukemia inhibitory factor is a glycoprotein growth and differentiation factor with pleiotropic activity. LIF has potent effects on the hematopoietic system, including megakaryocyte progenitor cells. In addition, LIF has bone regeneration activity, induces cachexia and acute-phase response in hepatocytes, and inhibits adipogenesis, to mention the more important activities. In vivo LIF treatment in monkeys and rodents was followed by signs of general toxicity, cachexia, acute-phase reaction, and stimulation of hematopoiesis. The safety margin for possible therapeutic effects on hematopoiesis seems to be very narrow.
生物活性
- Mouse leukemia inhibitory factor (LIF) inhibits proliferation of M-1 cells in a dose dependent manner. The expected IC50 for this effect is less than 0.15ng/ml.
电泳
- 1μg (R: reducing condition, N: non-reducing condition).
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