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产品中心 细胞因子 肿瘤坏死因子

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TNF-α Protein, Mouse STAR

TNF-α,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNF-a,Cachectin,DIF,TNFA,TNFSF2

价格 ￥800.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040059
规格
数量
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产品介绍

产品介绍 引用文献(1) 评论(0)

产品规格

  • 物种

    Mouse

  • 分子别名

    TNF-α,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNF-a,Cachectin,DIF,TNFA,TNFSF2

  • Accession

    P06804

  • 表达序列

    Leu80-Leu235

  • 表达宿主

    E.coli

  • 分子量

    17kDa

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <1EU/μg

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4, 1mM TCEP

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1、Hector J. et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-255.

    2、Berthold-Losleben M. et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.

背景介绍

  • Tumor Necrosis Factor alpha (TNF-α), is an inflammatory cytokine produced by macrophages/monocytes during acute inflammation and is responsible for a diverse range of signaling events within cells, leading to necrosis or apoptosis. TNF alpha exerts many of its effects by binding to either a 55 kDa cell membrane receptor termed. TNFα activates signals through two receptors, TNF-R1, which is expressed on most cell types, and TNF-R2, which is expressed mainly on immune cells. TNFα can have many functions including, to stimulate of phagocytosis in macrophages, to chemoattract neutrophils, to increase insulin resistance and to induce fever.

  • 生物活性

    • Measured in a cytotoxicity assay using NCTC clone 929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The expected IC50 < 50pg/ml.

  • 电泳

    • 1μg (R: reducing condition, N: non-reducing condition).

  • SPR

    • Protein A Chip captured TNFR-1/CD120a Fc Chimera, Human (Cat. No. UA040030), can bind TNF-α (80-235aa), Mouse (Cat. No. UA040059) with an affinity constant of 0.27nM as determined in SPR assay.

    • Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human (Cat. No. UA040028), can bind TNF-α(80-235aa), Mouse (Cat. No. UA040059) with an affinity constant of 1.24 nM as determined in SPR assay.

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