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物种
Human分子别名
Epigen,EPGN,EPG,Epigen precursorAccession
Q6UW88表达序列
Ala24-Ala95
表达宿主
E.coli分子量
8kDa
纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg活性
< 200ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 150mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1、Freed D M. et al. (2017) EGFR Ligands Differentially Stabilize Receptor Dimers to Specify Signaling Kinetics. Cell. 171(3):683-695.
2、Kochupurakkal B S. et al. (2005) Epigen, the last ligand of ErbB receptors, reveals intricate relationships between affinity and mitogenicity. J Biol Chem. 280(9): 8503-8512.
Epigen, human (EPGN) is a member of the epidermal growth factor family. EPGN is ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration. EPGN Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases. The precursor of EPG is a widely expressed transmembrane glycoprotein that undergoes cleavage at two sites to release a soluble EGF-like domain. EPGN might represent the last EGF-like growth factor and define a category of low affinity ligands, whose bioactivity differs from the more extensively studied high affinity ligands. Recombinant EPGN is expressed by Escherichia coli, composed of 72 (Ala24-Ala95) amino acids.
电泳
- 1μg (R: reducing condition, N: non-reducing condition).
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