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物种
Human分子别名
IL-2,interleukin-2Accession
P60568表达序列
Ala21-Thr153, with C-terminal His Tag
表达宿主
HEK293分子量
Theoretical: 17.1kDa Actual: 17,19kDa
纯度
>95% by SDS-PAGE
内毒素含量
<0.1EU/μg活性
Biological activity was measured in a cell proliferation assay using CTLL-2 cells. EC50<2.0ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
1*PBS, PH7.4, 5% trehalose
溶解方法
Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes. The major sources of IL-2 are activated CD4+ T cells and activated CD8+ T cells.IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. In the thymus, where T cells mature, it prevents autoimmune diseases by promoting the differentiation of certain immature T cells into regulatory T cells, which suppress other T cells that are otherwise primed to attack normal healthy cells in the body.
生物活性
- Biological Activity:measured in a cell proliferation assay using CTLL-2 cells. EC50<2.0ng/ml
电泳
- 2μg (R: reducing conditions)
ELISA
Immobilized human IL-2RA-Fc at 2μg/ml (50μL/well) can bind to IL-2 with a linear range of 0.17ng/ml to 40ng/ml.
Immobilized IL-2 His Tag, Human (Cat. No. UA040057) at 0.5μg/mL (100μL/well) can bind IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260) with EC50 of 1.23-1.99ng/ml.
SPR
Protein A Chip captured IL-2R alpha/CD25 Fc Chimera, Human (Cat. No. UA010260), can bind IL-2 His Tag, Human (Cat. No. UA040057) with an affinity constant of 15nM as determined in SPR assay.
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