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物种
Human分子别名
IL-6, B-Cell Stimulatory Factor 2, BSF-2, HSF, IFNB2, Interleukin-6, CTL Differentiation Factor, CDFAccession
P05231表达序列
Val30-Met212
表达宿主
E.coli标记
Unconjugated标签
No Tag缓冲体系
20mM Tris, 100mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1、Heinrich P C. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
2、Rose-John S. et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-624.
3、Dinh W. et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
| 项目 | 标准 |
|---|---|
| 性状（冻干粉、液体） | Lyophilized Powder |
| 支原体 | 阴性 |
| 无菌 | 符合 |
| pH值 | 7.7~8.3 |
| 蛋白含量 | 90%~120% |
| SDS-PAGE分子量 | 21 kD（还原） |
| SDS-PAGE纯度 | >97% |
| SEC-HPLC纯度 | >97% |
| 细胞活性 | < 0.2ng/ml |
Interleukin-6 (IL-6) is a member of the pro-inflammatory cytokine family, induces the expression of a variety of proteins responsible for acute inflammation, and plays an important role in the proliferation and differentiation of cells in humans. Interleukin 6 was originally identified as a T cell-derived lymphokine that induces final maturation of B cells into antibody-producing cells. Recombinant human IL-6 acts on B cells activated with Staphylococcus aureus Cowan I or pokeweed mitogen (PWM) to induce immunoglobulin M (IgM), IgG and IgA production, but not on resting B cells. Anti-IL-6 antibody was found to inhibit PWM-induced Ig production, indicating that IL-6 is one of the essential factors in PWM-induced Ig production. Furthermore, IL-6 was shown to augment the primary and secondary anti-SRBC (sheep red blood cell) antibody production in mice in vivo.
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