分享产品介绍 评论(0)
物种
Human分子别名
HBGF-4,HST Protein,HST-1,HSTF1,K-FGF,KFGFAccession
P08620表达序列
Ala31-Leu206
表达宿主
E.coli分子量
17kDa (Reducing)
纯度
＞95% by SDS-PAGE
内毒素含量
<1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 150mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Fibroblast Growth Factor 4, a secreted heparin-binding protein, is a member of the fibroblast growth factor (FGF) family. FGF4 plays an essential role in the midgut patterning as it inhibits the foregut development and promotes the midgut and hindgut identity. Thus FGF4 induces the intestinal fate, repressing anterior markers such as hematopoietically expressed homeobox 1 (HEX1) and enhancing midgut markers such as PDX1 and CDX2. Also, Wnt signaling is essential to the midgut and hindgut development; high Wnt activity is required to repress foregut development and push towards an intestinal fate. As an example, the a fore mentioned transcription factor BARX1, which is expressed during foregut embryogenesis, induces SFRP1 and SFRP2 which in turn inhibit Wnt signaling, thus repressing midgut fate and favoring the foregut fate. Another crucial transcription factor of intestinal specification is CDX2. CDX2 is expressed early during the anterior-posterior formation of the gut tube and is essential for specifying the intestinal endoderm. Wnt induces the expression of CDX2, thus reinforcing its predominant role in intestinal specification.
生物活性
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The EC50 for this effect is less than 0.2ng/mL.
电泳
- 1μg (R: reducing condition, N: non-reducing condition).
评论(0)