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物种
Human分子别名
G-CSF,CSF3,C17orf33,CSF3OS,GCSF,colony stimulating factor 3,CSF-3,Granulocyte Colony-Stimulating FactorAccession
P09919-2表达序列
Thr31-Pro204
表达宿主
E.coli分子量
19kDa
纯度
>95% by SDS-PAGE内毒素含量
<1EU/μg活性
<0.1ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 150mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-0.4 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1、Takano H. et al. (2007) G-CSF therapy for acute myocardial infarction. Trends Pharmacol Sci. 28(10): 512-517.
2、Klocke R. et al. (2008) Granulocyte colony-stimulating factor (G-CSF) for cardio- and cerebrovascular regenerative applications. Curr Med Chem. 15(10): 968-977.
3、Kang H J. et al. (2008) G-CSF- and erythropoietin-based cell therapy: a promising strategy for angiomyogenesis in myocardial infarction. Expert Rev Cardiovasc Ther. 6(5): 703-713.
4、Beekman R. et al. (2010) G-CSF and its receptor in myeloid malignancy. Blood. 115(25): 5131-6.
Granulocyte-colony stimulating factor (G-CSF) is a hematopoietic growth factor that stimulates the proliferation and differentiation of neutrophil precursor cells and enhances the functional properties of mature neutrophils. The mature human G-CSF (hG-CSF) is a glycoprotein with a molecular weight of approximately 19 kDa (174 amino acids) produced mainly by activated monocytes and macrophages. In the present scenario, hG-CSF has become one of the most widely used hematopoietic growth factor because of its proven efficacy against different forms of neutropenia caused mainly due to chemotherapy, radiotherapy and organ transplants. Mostly, it is recommended for the recovery from neutropenia after chemotherapy. The ability of G-CSF to mobilize stem cells from the bone marrow has found clinical applications in allogeneic and autologous transplantations. Also, the discovery of G-CSF as a neuroprotectant in neurological diseases like Alzheimer’s and Parkinson disease has garnered greater interests of researchers in the recent years.
生物活性
Measured in a cell proliferation assay using Murine M-NFS-60 cells, The EC50 for this effect is less than 0.1ng/ml.
电泳
- 1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized G-CSF, Human (Cat. No. UA040043) at 2.0μg/mL (100μL/well) can bind G-CSFR/CD114 Fc Chimera, Human (Cat. No. UA010537) with EC50 of 0.017-0.019μg/ml.
Immobilized G-CSF, Human (Cat. No. UA040043) at 2.0μg/mL (100μL/well) can bind G-CSFR/CD114 His Tag, Mouse (Cat. No. UA010219) with EC50 of 0.31-0.47μg/ml.
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