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物种
Human分子别名
APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta,CD253 antigen,CD253,Protein TRAIL,TL2,TNF-related apoptosis-inducing ligand,TNFSF10表达序列
Val 114-Gly281表达宿主
E.coli分子量
19-20kDa (Reducing)
纯度
＞95% by SDS-PAGE & RP-HPLC
内毒素含量
<1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 150mM NaCl, 1mM TCEP, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), also known as CD253 or TNFSF10, can be produced and secreted by most normal tissue cells. TRAIL proteins are available in two forms: membrane-bound (full-length TRAIL) and soluble (sTRAIL). In vivo, only the membrane-bound form exists, while the soluble polypeptide (sTRAIL), which is formed in the extracellular region of TRAIL, has the full biological activity of the membrane-bound TRAIL. Both of them are able to bind to specific receptors on the cell membrane and rapidly induce the expression of TRAIL through intracellular signal transduction. They can rapidly induce apoptosis in cells expressing TRAIL-specific receptors. TRAIL has a broad spectrum of action and can induce apoptosis in a variety of tumor cells in the colon, lung, breast, kidney, brain and skin, and is considered as a promising broad-spectrum anti-cancer molecule.
生物活性
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D，The EC50 for this effect is less than 0.8ng/ml.
电泳
- 1μg (R: reducing conditions, N: non-reducing conditions).
反相高效液相色谱(RP-HPLC)
ELISA
Immobilized TRAIL/Apo2L, Human (Cat. No. UA040039) at 2.0μg/mL (100μL/well) can bind TRAIL R1/TNFRSF10A Fc Chimera, Human (Cat. No. UA010630) with EC50 of 3.51-5.40 ng/mL.
SPR
Anti-His antibody Immobilized on CM5 Chip captured TNFRSF10B/TRAIL R2 His Tag, Mouse (Cat. No. UA010509), can bind TRAIL/Apo2L, Human (Cat. No. UA040039) with an affinity constant of 4.67nM as determined in SPR assay.
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