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物种
Human分子别名
Leukocyte interferon. B cell interferon,Type I interferon,IFNA2,IFN-a 2aAccession
P01563表达序列
Cys24-Glu188
表达宿主
E.coli分子量
17 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 150mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
Cytokine. 2011 Jan;53(1):52-9.
IFN-α is a cytokine that has an immunomodulatory function. It plays an important role not only in antiviral activity but also in several physiologic functions, such as activation of dendritic cells and accelerated expression of major histocompatibility complex I and II molecules that may cause increased antigen presentation. Variants of human leukocyte interferon α 2 (IFN-α 2a, α 2b, and α 2c) differ from each other by changes in their coding regions at nucleotide positions 137 and 170. IFN-α 2a is an important cytokine and used for antiviral and anticancer treatment.
免疫印迹
Primary antibody: IFIT1 Recombinant Rabbit mAb (S0B1242) at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 10 ng/ml IFN-α 2a(UA040038) for 16 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 58 kDa
生物活性
- Measured by a cytotoxicity assay using TF-1 Cells. The expected IC50 for this effect is less than 1ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% determined by RP-HPLC.
SPR
Protein A Chip captured IFN-alpha/beta R1 Fc Chimera Protein, Human (Cat. No. UA011186), can bind IFN-α 2a Protein, Human (Cat. No. UA040038) with an affinity constant of 1.30μM as determined in SPR assay.
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