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物种
Human抗原名称
IL-33Accession
O95760表达序列
Ser112-Thr270表达宿主
E.coli分子量
18-19kDa (Reducing)
纯度
＞95% by SDS-PAGE&RP-HPLC
内毒素含量
<1EU/μg活性
<2ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
50mM Tris, 50mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
IL-33 is a nuclear cytokine from the IL-1 family constitutively expressed in epithelial barrier tissues and lymphoid organs, which plays important roles in type-2 innate immunity and human asthma. Recent studies indicate that IL-33 induces production of large amounts of IL-5 and IL-13 by group 2 innate lymphoid cells (ILC2s), for initiation of allergic inflammation shortly after exposure to allergens or infection with parasites or viruses. IL-33 appears to function as an alarmin (alarm signal) rapidly released from producing cells upon cellular damage or cellular stress.
生物活性
- Measured in a cell proliferation assay D10.D4.1 cells, the EC50 for this effect is less than 2ng/ml.
电泳
- 1μg (R: reducing conditions, N: non-reducing conditions).
反相高效液相色谱(RP-HPLC)
ELISA
Immobilized IL-33, Human (Cat. No. UA040036) at 2.0μg/mL (100μL/well) can bind IL1RL1/ST2 Fc Chimera, Human (Cat. No. UA010464) with EC50 of 4.42-4.97 ng/mL.
SPR
Protein A Chip captured IL1RL1/ST2 Fc Chimera, Human (Cat. No. UA010464), can bind IL-33, Human (Cat. No. UA040036) with an affinity constant of 1.25nM as determined in SPR assay .
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