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TNFR-1/CD120a Protein, Human
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规格：100μg
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产品介绍 评论(0)
物种
HumanAccession
P19438表达序列
Leu30-Thr211, with C-terminal Human IgG1 Fc表达宿主
HEK293分子量
55-70kDa (Reducing)
纯度
>95% by SDS-PAGE&RP-HPLC
内毒素含量
<0.1EU/μg标记
Unconjugated标签
Human Fc Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
TNFR-1 is a member of the TNF receptor superfamily of proteins which is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mice lacking a functional copy of this gene exhibit impaired immune function.
生物活性
- Measured by its ability to inhibit the TNF-α mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The EC50 for this effect is less than 1.5ng/mL in the presence of 0.25 ng/mL Recombinant Human TNF‑ α.
电泳
- 2μg (R: reducing conditions, N: non-reducing conditions).
反相高效液相色谱(RP-HPLC)
ELISA
Immobilized TNF-α Protein, Human (Cat. No. UA040018) at 0.2μg/mL (100μL/well) can bind TNFR-1/CD120a Protein, Human (Cat. No. UA040030) with EC50 of 1.90-2.41ng/mL.
SPR
Protein A Chip captured TNFR-1/CD120a Fc Chimera, Human (Cat. No. UA040030), can bind TNF-α (80-235aa), Mouse (Cat. No. UA040059) with an affinity constant of 0.27nM as determined in SPR assay.
Protein A Chip captured TNFR-1/CD120a Fc Chimera, Human (Cat. No. UA040030), can bind TNF-α His Tag, Mouse (Cat. No. UA040083) with an affinity constant of 0.069nM as determined in SPR assay.
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