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产品中心 细胞因子 白细胞介素

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IL-4 Protein, Human STAR

价格 ￥1,880.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040026
规格
数量
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产品介绍

产品介绍 引用文献(4) 评论(0)

产品规格

  • 物种

    Human

  • 抗原名称

    IL-4

  • Accession

    P05112

  • 表达序列

    His25-Ser153

  • 表达宿主

    E.coli

  • 分子量

    15-16kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 活性

    <0.2ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

背景介绍

  • Interleukin 4(IL4) was first identified as a helper T cell product with the capacity to co-stimulate B cell growth in vitro. IL-4 also can rescue B-cells from apoptosis, enhancing their survival, and is responsible for immunoglobulin isotype switching to IgG1 and IgE. The effect of IL-4 signaling is mediated through the IL-4 receptor alpha chain (IL-4Rα). Upon binding to its ligand, IL-4Rα dimerizes either with the common gamma chain (γc) to produce the type-1 signaling complex located mainly on hematopoietic cells, or with the IL-13 receptor alpha 1 (IL-13Rα1) to produce the type-2 complex, which is expressed also on non-hematopoietic cells. The type-1 signaling complex is critical for Th2-skewing of T cells and the development of alternatively activated macrophages (AAMΦs), while the type-2 complex plays a role in non-hematopoietic responses to IL-4 and IL-13.

  • 免疫印迹

    • Primary antibody: CD209 gamma Recombinant Rabbit mAb (S0B1390) at 1/1000 dilution

      Lane 1: untreated THP-1 whole cell lysate 20 µg

      Lane 2: THP-1 treated with 100 ng/ml GM-CSF (UA040002) and 50 ng/ml hIL-4 (UA040026) for 7 days whole cell lysate 20 µg

      Lane 3: THP-1 treated with 100 ng/ml GM-CSF (Competotor T) and 50 ng/ml hIL-4 (UA040026) for 7 days whole cell lysate 20 µg

      Lane 4: THP-1 treated with 100 ng/ml GM-CSF (UA040002) and 50 ng/ml hIL-4 (Competitor P) for 7 days whole cell lysate 20 µg

      Lane 5: THP-1 treated with 100 ng/ml GM-CSF (Competotor T) and 50 ng/ml hIL-4 (Competitor P) for 7 days whole cell lysate 20 µg

      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

      Predicted MW: 46 kDa

      Observed MW: 45~55 kDa

  • 生物活性

    • Measured in a cell proliferation assay using TF-1 human erythroleukemic cells, the EC50 for this effect is less than 0.2ng/ml.

    • Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is less than 0.2ng/ml.

  • 电泳

    • 1μg (R: reducing condition, N: non-reducing condition).

  • ELISA

    • Immobilized IL-4 Protein, Human (Cat. No. UA040026) at 2.0μg/mL (100μL/well) can bind IL-4R alpha/CD124 Fc Chimera Protein, Cynomolgus (Cat. No. UA010387) with EC50 of 1.97-3.24 ng/mL.

    • Immobilized IL-4, Human (Cat. No. UA040026) at 2.0μg/mL (100μL/well) can bind IL-4Rα/CD124 His Tag, Cynomolgus (Cat. No. UA010379) with EC50 of 42.22-60.87 ng/mL.

    • Immobilized IL-4 Protein, Human (Cat. No. UA040026) at 5.0μg/mL (100μL/well) can bind IL-4R alpha/CD124 His Tag Protein, Human (Cat. No. UA010382) with EC50 of 13.41-18.20ng/mL.

    • Immobilized IL-4 Protein, Human (Cat. No. UA040026) at 5.0μg/mL (100μL/well) can bind Biotinylated IL-4R alpha/CD124 Avi&His Tag Protein, Human (Cat. No. UA010671) with EC50 of 27.67-45.87ng/mL.

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