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产品中心 细胞因子 趋化因子及其受体

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RSPO3 Protein, Human STAR

R-spondin 3

价格 ￥860.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040025
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    R-spondin 3

  • Accession

    Q9BXY4-1

  • 表达序列

    Gln22-Val146, with C-terminal 8*His

  • 表达宿主

    HEK293

  • 分子量

    16 and 20-24kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, -20 to -70 °C as supplied.
    · 6 months, -20 to -70 °C under sterile conditions after reconstitution.
    · 1 week, 2 to 8 °C under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

背景介绍

  • R-spondin3 (RSPO3), an activator of Wnt/β-catenin signaling, plays a key role in tumorigenesis of various cancers. RSPO3 contains two adjacent cysteine-rich furin-like domains (aa 35-135) with one potential N-glycosylation site (aa 36), followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-269). RSPO3 is a novel contraction-inducible myokine produced by cultured human myotubes. Human RSPO3 is a paracrine factor that may positively participate in the myogenesis processes of myoblasts and satellite cells. RSPO3 promotes the tumor growth of choriocarcinoma via Akt/PI3K/ERK signaling, which supports RSPO3 as an oncogenic driver promoting the progression of choriocarcinoma. RSPO3 plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this protein with bone mineral density and risk of fracture.

  • 生物活性

    • Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The EC50 for this effect is less than 50ng/mL in the presence of 5ng/mL Recombinant Human Wnt‑3a.

  • 电泳

    • 2μg (R: reducing conditions, N: non-reducing conditions).

  • ELISA

    • Immobilized RSPO3 His Tag, Human (Cat. No. UA040025) at 2.0μg/mL (100μL/well) can bind ZNRF3 Fc Chimera Protein, Human (Cat. No. UA011119) with EC50 of 1.59-3.16 ng/mL.

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