热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 细胞因子 生长因子

所有图片（1/3）

LIF Protein, Human

价格 ￥950.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040023
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • Accession

    P15018

  • 表达序列

    Ser23-Phe202

  • 表达宿主

    E.coli

  • 分子量

    19-20kDa (Non-reducing)

  • 纯度

    ＞95%by SDS-PAGE&RP-HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50<0.3ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM Tris, 200mM NaCl, pH8.0

  • 溶解方法

    Reconstitute at 0.5-1mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

背景介绍

  • Leukaemia inhibitory factor (LIF) is produced by many different cell types and has pleiotropic actions. LIF stimulates the differentiation of the macrophage cell line M1, and the proliferation of haematopoietic stem cells. In vivo, it has profound effects on haematopoiesis, particularly in combination with other cytokines such as IL-3, causing increased platelet formation. LIF allows embryonic stem cells to remain in an undifferentiated state and can maintain their proliferation in culture. LIF stimulates synthesis of acute phase proteins by liver cells, increases bone resorption, stimulates differentiation of cholinergic nerves and loss of body fat.

  • 生物活性

    • Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The EC50 for this effect is less than 0.3ng/ml.

  • 电泳

    • 2μg（N: non-reducing conditions).

  • 反相高效液相色谱(RP-HPLC)

评论(0)