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物种
Human抗原名称
R-Spondin 1分子别名
RSPO1,CRISTIN3,FLJ40906,RP11-566C13.1,R-spondin-1Accession
Q2MKA7-1表达序列
Ser21-Ala146, with C-terminal 8*His
表达宿主
HEK293分子量
17-25kDa (Reducing)
纯度
>95% by SDS-PAGE
内毒素含量
<0.1EU/μg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway. Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer. RSPO1 stands out among RSPOs molecules with respect to its potential therapeutic use, especially in the Regenerative Medicine field,due to its mitogenic activity in stem cells. Here, we generated a recombinant human RSPO1 (rhRSPO1) using the HEK293 cell line, obtaining a purified, characterized and biologically active protein product to be used in Cell Therapy.
生物活性
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The EC50 for this effect is less than 100ng/mL in the presence of 5ng/mL Recombinant Human Wnt‑3a.
电泳
- 2μg (R: reducing conditions, N: non-reducing conditions).
SPR
RNF43 mFc Chimera, Mouse (Cat. No. UA010216) captured on Protein A Biosenor, can bind RSPO1(21-146) His Tag, Human (Cat. No. UA040022) with an affinity constant of 6.73nM as determined in SPR assay.
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