TNF-α Protein, Human STAR

价格￥460.00 （供应商现货： 3-5个工作日）

货号 UA040018

数量

规格：10μg

价格：￥460.00

产品介绍

产品介绍引用文献(1) 评论(0)

产品规格

物种

Human
Accession

P01375
表达序列

Val77-Leu233
表达宿主

E.coli
分子量

17kDa (Reducing)
纯度

＞95% by SDS-PAGE
内毒素含量

<1EU/μg
标记

Unconjugated
标签

No Tag
性状

Lyophilized powder
缓冲体系

PBS, pH7.4
溶解方法

Reconstitute at 0.1-1mg/mL according to the size in ultrapure water after rapid centrifugation.
储存条件

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.

背景介绍

Tumor Necrosis Factor alpha (TNF-α), is an inflammatory cytokine produced by macrophages/monocytes during acute inflammation and is responsible for a diverse range of signaling events within cells, leading to necrosis or apoptosis. TNF alpha exerts many of its effects by binding to either a 55 kDa cell membrane receptor termed. TNFα activates signals through two receptors, TNF-R1, which is expressed on most cell types, and TNF-R2, which is expressed mainly on immune cells. TNFα can have many functions including, to stimulate of phagocytosis in macrophages, to chemoattract neutrophils, to increase insulin resistance and to induce fever.

免疫印迹
- Primary antibody: Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa treated with 100 ng/ml Calyculin A for 30 minutes, whole cell lysate 20 µg
  Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 65 kDa
生物活性
- Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, ED50 < 40pg/ml.
- Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less than 10pg/ml.
电泳
- 1μg（N: non-reducing condition, R: reducing condition).
ELISA
- Immobilized TNF-α Protein, Human (Cat. No. UA040018) at 0.2μg/mL (100μL/well) can bind TNFR-1/CD120a Protein, Human (Cat. No. UA040030) with EC50 of 1.90-2.41ng/mL.
SPR
- Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human (Cat. No. UA040028), can bind TNF-α, Human (Cat. No. UA040018) with an affinity constant of 0.23 nM as determined in SPR assay.
- Anti-His antibody Immobilized on CM5 Chip captured TNFR-2/CD120b His Tag, Human (Cat. No. UA010138), can bind TNF-α, Human (Cat. No. UA040018) with an affinity constant of 0.71 nM as determined in SPR assay.

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