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物种
HumanAccession
P01127表达序列
Ser82-Thr190
表达宿主
Yeast分子量
33-40 kDa(Non-reducing)
纯度
＞95% by SDS-PAGE
内毒素含量
<0.2EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
10 mM Acetic acid, pH3.3
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Platelet-derived growth factor (PDGF) is a family of three disulfide-linked glycoprotein dimers of M.W. 29kDa-32kDa that arise from astochastic assembly of the homologous subunits, A-chain and B-chain, yielding the heterodimer PDGF-AB and homodimers PDGF-AA and PDGF-BB. The B-chain contains a cationic carboxyl terminus that has been implicated in the strong association of PDGF-BB with the cell surface and wound healing.
免疫印迹
Primary antibody: Phospho-SHP-2 (Tyr542) Recombinant Rabbit mAb(S0B1093) at 1/5000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 starve 18 hours, then treated with 50 ng/ml PDGF-BB(Competitor R) for 20 minutes whole cell lysate 20 µg
Lane 3: NIH/3T3 starve 18 hours, then treated with 50 ng/ml PDG-BB(UA040014) for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 68 kDa
生物活性JSON
Measured by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells, EC50 < 3.0 ng/ml.
电泳JSON
2μg（R: reducing conditions, N: non-reducing conditions).
ELISA
Immobilized PDGF-BB Protein, Human (Cat. No. UA040014) at 1.0μg/mL (100μL/well) can bind PDGF R alpha/PDGFRA Fc Chimera Protein, Human (Cat. No. UA010718) with EC50 of 23.57-26.73ng/mL.
Immobilized PDGF-BB Protein, Human (Cat. No. UA040014) at 1.0μg/mL (100μL/well) can bind PDGF R alpha/PDGFRA His Tag Protein, Human (Cat. No. UA010720) with EC50 of 0.19-0.26μg/mL.
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