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产品中心 细胞因子 生长因子

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FGF-basic(154aa) Protein, Human STAR

bFGF,FGF-2,Heparin-binding growth factor 2,Basic fibroblast growth factor,FGFB,HBGF-2,FGF2,Fibroblast Growth Factor 2 (Basic)

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040007
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    bFGF,FGF-2，Heparin-binding growth factor 2，Basic fibroblast growth factor，FGFB,HBGF-2，FGF2，Fibroblast Growth Factor 2 (Basic)

  • Accession

    P09038

  • 表达序列

    Ala135-Ser288

  • 表达宿主

    E.coli

  • 分子量

    17-18kDa(Reducing)

  • 纯度

    ＞95%, by SDS-PAGE under reducing conditions

  • 内毒素含量

    <1EU/μg

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM PB, 500mM NaCl, pH7.4

  • 溶解方法

    Reconstitute at 0.5 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

背景介绍

  • Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.

  • 生物活性

    • Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells.The EC50 for this effect is less than 0.1ng/ml.corresponding to a specific activity of > 1 x 107 units/mg.

    • Progression of cerebral organoid development from human PSCs.

    • Staining for brain regions and neuronal cell identities.
      Staining for neurons (TUJ1, purple) and progenitors (SOX2, orange) in a large continuous cortical tissue within an organoid. A forebrain region of an organoid staining positive for the marker FOXG1 (green).

  • 电泳

    • FGF-basic(154aa), Human ，1μg（R: reducing conditions, N: non-reducing conditions).

  • ELISA

    • Immobilized FGF-basic(154aa), Human (Cat. No. UA040007) at 1μg/mL (100μL/well) can bind FGF-R4/CD334 His Tag, Human (Cat. No. UA020009) with EC50 of 0.968μg/mL.

    • Immobilized FGF-basic(154aa), Human (Cat. No. UA040007) at 2.5μg/mL (100μL/well) can bind Human FGF-R1/CD331, His Tag (Cat. No. UA020008) with EC50 of 0.049-0.066μg/ ml.

    • Immobilized FGF-basic(154aa) Protein, Human (Cat. No. UA040007) at 2.0μg/mL (100μL/well) can bind Glypican 1/GPC1 His Tag Protein, Human (Cat. No. UA011160) with EC50 of 17.56-25.93ng/mL.

    • Immobilized FGF-basic(154aa) Protein, Human (Cat. No. UA040007) at 2.0μg/mL (100μL/well) can bind GPC3 Fc Chimera Protein, Human (Cat. No. UA010680) with EC50 of 3.97-7.23ng/mL.

    • Immobilized FGF-basic(154aa) Protein, Human (Cat. No. UA040007) at 2.0μg/mL (100μL/well) can bind GPC3 His Tag Protein, Human (Cat. No. UA010073) with EC50 of 11.98-19.94ng/mL.

  • SPR

    • Anti-His antibody Immobilized on CM5 Chip captured FGF-R1/CD331 His Tag, Human (Cat. No. UA020008), can bind FGF-basic(154aa), Human (Cat. No. UA040007) with an affinity constant of 0.33μM as determined in SPR assay.

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