TNF-α Protein, Human
TNF-alpha,Tumor necrosis factor,Cachectin,Tumor necrosis factor ligand superfamily member 2,TNF-a,TNF,TNFA,TNFSF2,TNF-α,TNFα,TNF alpha,TNFalpha,Tumor Necrosis Factor,TNF-Alpha,Tumor Necrosis Factor Ligand Superfamily Member 2
货号： UA040005
- 价格： ￥460
- 规格：
- 数量：
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物种
Human分子别名
TNFα;Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFA, TNFSF2Accession
P01375表达序列
Val77-Leu233
表达宿主
CHO分子量
17 kDa(Reducing)
纯度
＞95%, by SDS-PAGE under reducing conditions内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Tumor necrosis factor alpha (TNFa) is a multifunctional proinflammatory cytokine synthesized mainly by nucleated blood
cells as a 233 aa type II transmembrane protein which is cleaved by ADAM17 between aa 76-77 to form a soluble
homotrimeric complex. TNFa plays a role in lipid metabolism, coagulation, and endothelial function and has been
associated with cancer, infection and inflammation (including inflammatory bowel disease), ischemia/reperfusion injury
and heart failure, and insulin resistance.
免疫印迹
Primary antibody: Phospho-NF-κB p65 (Ser536) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa treated with 100 ng/ml Calyculin A for 30 minutes, whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 65 kDa
生物活性JSON
EC50 < 0.1ng/ml, measured in a cytotoxicity assay using L-929.
Immobilized TNF-α, Human (Cat. No. UA040005) at 10μg/ml (100μl/well) can bind TNFR-1/CD120a His Tag, Human (Cat. No. UA040028) with EC50 of 0.167 μg/ml.
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, EC50 < 40pg/ml.
电泳JSON
TNF-α, Human，2μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.
SPR
Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human (Cat. No. UA040028), can bind TNF-α, Human (Cat. No. UA040005) with an affinity constant of 0.106nM as determined in SPR assay.
Anti-His antibody Immobilized on CM5 Chip captured TNFR-2/CD120b His Tag, Human (Cat. No. UA010138), can bind TNF-α, Human (Cat. No. UA040005) with an affinity constant of 1.53 nM as determined in SPR assay.
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