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EGF Protein, Human

货号： UA040003

Datasheet COA
  • 价格： ￥148
  • 规格：
  • 数量：
斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • Accession

    P01133

  • 表达序列

    Asn971-Arg1023, with an N-terminal Met

  • 表达宿主

    E.coli

  • 分子量

    7kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.2EU/μg

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 稀释度

背景介绍

  • Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Epidermal growth factor (EGF) binds to epidermal growth factor receptor and stimulates an intracellular signal transduction cascade, leading to activation of genes that regulate cell proliferation, angiogenesis, motility, and metastasis. Epidermal growth factor (EGF) mRNA and protein are expressed in a number of adult tissues, especially in epithelial cells in the gastrointestinal tract. Predominant sites of synthesis of this peptide are the submandibular glands, the Brunner glands in the small intestine and the kidney.

  • 免疫印迹

    • Primary antibody: Phospho-Stat5 (Tyr694) Recombinant Rabbit mAb(S0B0851) at 1/1000 dilution

      Lane 1: untreated A431 whole cell lysate 20 µg

      Lane 2: A431 treated with 100 ng/ml EGF(UA040003) for 5 minutes whole cell lysate 20 µg

      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

      Predicted MW: 91 kDa

      Observed MW: 90 kDa

  • 生物活性

    • The EC50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2 ng/ml.

  • 电泳

    • 2μg（R: reducing conditions, N: non-reducing conditions).

  • ELISA

    • Immobilized EGF, Human (Cat. No. UA040003) at 0.5μg/mL (100μL/well) can bind EGFR Fc Chimera, Human (Cat. No. UA010174) with EC50 of 0.11-0.15μg/ml.

    • Immobilized EGF, Human (Cat. No. UA040003) at 1.0μg/mL (100μL/well) can bind EGFR His Tag, Rhesus macaque (Cat. No. UA010168) with EC50 of 0.54-1.0μg/ ml.

    • Immobilized EGF, Human (Cat. No. UA040003) at 1.5μg/mL (100μL/well) can bind EGFR His Tag, Mouse (Cat. No. UA010161) with EC50 of 0.84-1.44μg/ ml.

  • SPR

    • CM5 Chip captured EGF, Human (Cat. No. UA040003), can bind EGFR His Tag, Human (Cat. No. UA010306) with an affinity constant of 2.01μM as determined in SPR assay.

    • CM5 Chip captured EGF, Human (Cat. No. UA040003), can bind EGFR His Tag, Rhesus macaque (Cat. No. UA010168) with an affinity constant of 0.53mM as determined in SPR assay.

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