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分子别名
Envelope glycoprotein E,gE,HHV-3 gE,glycoprotein E ProteinAccession
Q9J3M8表达序列
Ser31-Tyr538表达宿主
HEK293分子量
58-82kDa(Reducing)
纯度
＞95% by SDS-PAGE & RP-HPLC活性
Immobilized VZVgE Flag Tag Protein (Cat. No. UA030073) at 2.0μg/mL (100μL/well) can bind Varicella-zoster virus (VZV) / HHV-3 gE / glycoprotein E Antibody, Mouse MAb with EC50 of 1.22-2.62ng/mL.标记
Unconjugated标签
Flag Tag性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
Julie K. Olson, Richard A. Santos, Charles Grose, Varicella-Zoster Virus Glycoprotein gE: Endocytosis and Trafficking of the Fc Receptor, The Journal of Infectious Diseases, Volume 178, Issue Supplement_1, November 1998, Pages S2–S6.
Varicella-zoster virus (VZV) serves as the prototype for the alphaherpesvirus subgroup, classified under the Varicellovirus genus. The VZV genome encodes a minimum of six glycoproteins: gB, gC, gE, gH, gI, and gL. Among these, gE (formerly known as gpI or gp98) is the most abundant in the virion envelope and is the primary glycoprotein present on the surface of infected cells.
VZV gE is a classic type I transmembrane glycoprotein, extensively modified by N-linked and O-linked glycosylation, sialylation, and sulfation. It also features a phosphorylation site for serine/threonine casein kinase II. Additionally, gE has the capacity to form homodimers that undergo tyrosine phosphorylation.
During infection, VZV gE interacts with VZV gI to form a protein complex. Notably, gE on the surface of infected cells has been identified as an Fc receptor for nonimmune IgG. Homology studies indicate that gE exhibits structural characteristics akin to those of several nonviral cell surface receptors, such as the Fc receptor (FcγRII) and the low-density lipoprotein (LDL) receptor.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
The purity of VZVgE Flag Tag Protein is more than 95% determined by RP-HPLC.
ELISA
Immobilized VZVgE Flag Tag Protein (Cat. No. UA030073) at 2.0μg/mL (100μL/well) can bind Varicella-zoster virus (VZV) / HHV-3 gE / glycoprotein E Antibody, Mouse MAb with EC50 of 1.22-2.62ng/mL.
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