HRSVB(Strain 18537）Nucleoprotein ,His tag Protein

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货号： UA030038

大包装询价

产品介绍引用文献(0) 评论(0)

产品规格

物种

HRSV
分子别名

Nucleoprotein, Protein N, Nucleocapsid protein
Accession

P24566
表达序列

Met1-Leu391, with N-terminal 8*His MHHHHHHHHMALSKVKLNDTLNKDQLLSSSKYTIQRSTGDNIDTPNYDVQKHLNKLCGMLLITEDANHKFTGLIGMLYAMSRLGREDTIKILKDAGYHVKANGVDITTYRQDINGKEMKFEVLTLSSLTSEIQVNIEIESRKSYKKLLKEMGEVAPEYRHDSPDCGMIILCIAALVITKLAAGDRSGLTAVIRRANNVLKNEIKRYKGLIPKDIANSFYEVFEKHPHLIDVFVHFGIAQSSTRGGSRVEGIFAGLFMNAYGSGQVMLRWGVLAKSVKNIMLGHASVQAEMEQVVEVYEYAQKLGGEAGFYHILNNPKASLLSLTQFPNFSSVVLGNAAGLGIMGEYRGTPRNQDLYDAAKAYAEQLKENGVINYSVLDLTAEELEAIKHQLNPKEDDVEL
表达宿主

E.coli
分子量

43kDa
纯度

>95% by SDS-PAGE
内毒素含量

<1EU/μg
标记

Unconjugated
标签

His Tag
性状

Lyophilized powder
缓冲体系

20mM PB, 150mM NaCl, 1mM EDTA, pH7.4
溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用

1、Oliveira A P. et al. (2013) Human respiratory syncytial virus N, P and M protein interactions in HEK-293T cells. Virus Res. 177(1): 108-112.
2、Céspedes P F. et al. (2014) Surface expression of the hRSV nucleoprotein impairs immunological synapse formation with T cells. Proc Natl Acad Sci U S A. 111(31): E3214.

背景介绍

Human Respiratory Syncytial Virus (HRSV) is a negative-sense single-stranded RNA virus that belongs to the Paramyxoviridae family and the Pneumovirinae subfamily, and considered the most important pathogen causing respiratory disease in infants and neonates worldwide. The nucleoprotein of HRSV, a canonical cytosolic protein, is highly conserved among pneumoviruses and interacts with viral RNA, generating a helicoidal nucleocapsid which is responsible for genome and anti-genome resistance to RNase. In addition, the nucleoprotein could be responsible for inhibiting T-cell activation during infection by HRSV, and expressed on the surface of epithelial and dendritic cells, preventing immunological synapse assembly by naïve CD4+ T cells. Therefore, this protein is defined as a major virulence factor that contributes to impairing acquired immunity and enhances susceptibility to reinfection by HRSV.