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SerpinB3/SCCA Protein, Human

SerpinB3,SCCA1,Serpin B3,Protein T4-A,Squamous cell carcinoma antigen 1,SCCA-1,serine (or cysteine) proteinase inhibitor,clade B (ovalbumin),member 3,serpin peptidase inhibitor,T4-A

价格 ￥1,100.00 （ 供应商现货 ： 3-5个工作日）
货号 UA030001
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    SerpinB3, SCCA1;Serpin B3, Protein T4-A, Squamous cell carcinoma antigen 1, SCCA-1,serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3, serpin peptidase inhibitor, clade B (ovalbumin), member 3, Squamous cell carcinoma antigen 1, T4-A,SCCA1

  • Accession

    P29508

  • 表达序列

    MHHHHHHDDDDKNSLSEANTKFMFDLFQQFRKSKENNIFYSPISITSALGMVLLGAKDNTAQQIKKVLHFDQVTENTTGKAATYHVDRSGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYLFLQEYLDAIKKFYQTSVESVDFANAPEESRKKINSWVESQTNEKIKNLIPEGNIGSNTTLVLVNAIYFKGQWEKKFNKEDTKEEKFWPNKNTYKSIQMMRQYTSFHFASLEDVQAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETRVDLHLPRFKVEESYDLKDTLRTMGMVDIFNGDADLSGMTGSRGLVLSGVLHKAFVEVTEEGAEAAAATAVVGFGSSPTSTNEEFHCNHPFLFFIRQNKTNSILFYGRFSSP

  • 表达宿主

    E.coli

  • 分子量

    45.9 kDa

  • 纯度

    ＞90%, by SDS-PAGE under reducing conditions

  • 内毒素含量

    <2EU/μg

  • 活性

    Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 is 5.2 nM, as measured under the described conditions.

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM Tris-HCl, 150mM NaCl, pH8.0

  • 溶解方法

    Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

背景介绍

  • SerpinB3/SCCA belongs to tumor-related glycoprotein antigen, also known as TA-4 antigen, which widely exists in the cytoplasm of uterine, cervical, lung and other squamous cell carcinoma, and has a high content in non-keratinized cancer cells, which is closely related to the occurrence and development of squamous cell carcinoma. In normal squamous epithelial cells, SerpinB3/SCCA inhibits apoptosis and participates in the differentiation of squamous epithelium, but participates in the physiological processes such as invasion, metastasis and recurrence of cancer cells in tumor cells. As a specific marker of squamous cell carcinoma, the concentration of SerpinB3/SCCA increases with the aggravation of the disease, and it is an important tumor marker reflecting the biological characteristics of squamous cell carcinoma. Its serum level is widely used in the diagnosis of squamous cell carcinoma of many organs and clinical evaluation of therapeutic effect.

操作步骤

  • 实验方法
    实验原理：检测其抑制活性Cathepsin L蛋白裂解荧光素肽底物Z-LR-AMC的能力。
    实验材料
    1.检测缓冲液：50 mM MES, 5 mM DTT, pH 6.0
    2.SerpinB3/SCCA Protein, Human
    3.Recombinant Human Cathepsin L
    4.底物：Z-Leu-Arg-AMC (Catalog # ES008)
    5.96 ELISA Removable Plate, Black, High binding (GENEVER, Catalog # GMO2-96H)
    6.读板器（PerkinElmer，激发波长380 nm，发射波长460 nm）
    实验步骤
    1.在检测缓冲液中将Cathepsin L蛋白稀释至40 µg/mL。
    2.冰上孵育15分钟。
    3.在检测缓冲液中将激活后的Cathepsin L蛋白稀释至0.2 µg/mL。
    4.使用检测缓冲液对SerpinB3蛋白梯度稀释：起始浓度1000nM, 3倍稀释，11个浓度点（包含0）。
    5.取25 μL各稀释浓度的Serpin B3蛋白与25 μL 0.2 μg/mL的Cathepsin L蛋白混合。
    6.37°C孵育15分钟。
    7.每管加入200 μL检测缓冲液稀释反应液。
    8.在检测缓冲液中稀释底物至 20 µM。
    9.在黑色孔板中加入50 µL的反应混合物，然后加入50 µL的20 µM底物以启动反应。同时设置仅含检测缓冲液和底物的对照组。
    10.在激发和发射波长分别为380 nm和460 nm下，以动力学模式读取信号10分钟。
    11.计算特异性活性：

    Specific Activity (pmol/min/µg) =

    Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)

    amount of enzyme (µg)

    *底物空白校正
    **使用校准标准品7-氨基-4-甲基香豆素（7-amino-4-Methyl Coumarin）推导。

  • 电泳

    • SerpinB3/SCCA1, Human，1μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 90%.

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