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产品中心 药物靶点和Fc受体 药物靶点

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CD73 hFc Tag Protein, Human

5'-nucleotidase,5'-NT,5'-deoxynucleotidase,Ecto-5'-nucleotidase,IMP-specific 5'-nucleotidase,Thymidylate 5'-phosphatase,NT5E,NT5,NTE

价格 ￥1,100.00 （ 1-2周）
货号 UA019051
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase, NT5E, NT5, NTE

  • Accession

    P21589

  • 表达序列

    Protein sequence (P21589, Trp27-Ser549, with C-hFc tag) WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFS

  • 表达宿主

    HEK293

  • 分子量

    Predicted MW: 84.1 kDa Observed MW: 94 kDa

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    Human Fc Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.

  • 溶解方法

    Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.

  • 储存条件

    12 months from date of receipt, -20 to -70 °C as supplied.
    6 months, -20 to -70 °C under sterile conditions after reconstitution.
    1 week, 2 to 8 °C under sterile conditions after reconstitution.
    Please avoid repeated freeze-thaw cycles.

背景介绍

  • 5′-nucleotidase (5′-NT) also known as CD73, is an enzyme that in humans is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). NT5E can act as an immune inhibitory control molecule. Free adenosine generated by NT5E inhibits cellular immune responses and thereby promotes immune escape of tumor cells. Due to enzymatic and non-enzymatic properties, CD73 is involved in cancer-related processes and is upregulated in many cancers such as leukemia, glioblastoma, melanoma, oesophageal, prostate, ovarian and breast cancer. It is an important key molecule in cancer regulation and development and is involved in tumor progression. In addition, NT5E functions as an adhesion and signaling molecule and can regulate cellular signaling with extracellular matrix components such as fibronectin and laminin.

  • 生物活性

    • Immobilized Human CD73 Protein, hFc Tag at 1 μg/mL (50 μL/well) can bind CD73 Recombinant Rabbit mAb (SDT-218-52) (Cat. No. S0B2139) with EC50 of 7.061-10.29 ng/ml.

  • 电泳

    • 2 μg（R: reducing conditions）

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