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产品中心 药物靶点和Fc受体 药物靶点

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LGR5/GPR49 His Tag Protein, Cynomolgus

LGR5,GPR49,GPR67

价格 ￥1,290.00 （ 供应商现货 ： 3-5个工作日）
货号 UA016188
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    LGR5, GPR49, GPR67

  • Accession

    G7PI19

  • 表达序列

    Ser22 - Gly557 with His Tag at the C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    70-100kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 活性

    Immobilized Human R-Spondin1/RSPO1 Protein, Human (Cat. No. UA040347) at 5 μg/mL (100 μL/well) can bind LGR5 / GPR49 His Tag Protein, Cynomolgus (Cat. No. UA016188) with a linear range of 8.6-10.06 ng/mL (Routinely tested).

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Peng, W.C., et al. (2013). Structure of stem cell growth factor R-spondin 1 in complex with the ectodomain of its receptor LGR5. Cell Reports, 3(6), 1885-1892.
    2. Carmon, K.S., et al. (2011). LGR4 and LGR5 are R-spondin receptors mediating Wnt/β-catenin and Wnt/PCP signalling. EMBO Reports, 12(10), 1055-1061.
    3. de Lau, W., et al. (2011). LGR5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature, 476(7360), 293-297.

背景介绍

  • Cynomolgus macaque (Macaca fascicularis) LGR5 is encoded by the LGR5 gene (Gene ID: 102120216) located on chromosome 11. The reference sequence is XM_005571542.4, encoding isoform X1 with protein accession XP_005571599.2. The cynomolgus LGR5 UniProt/TrEMBL accession is A0A2K5WIN4.

    Cynomolgus LGR5 is highly homologous to human LGR5 in both structure and function. Recombinant cynomolgus LGR5 protein typically covers the extracellular domain spanning Ser22-Gly557 (corresponding to human LGR5 residues 22-543), with a C-terminal His tag. The protein is expressed in HEK293 cells and exhibits biological activity in binding human R-spondin 1.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • The purity of LGR5 / GPR49 His Tag Protein, Cynomolgus is more than 95% determined by SEC-HPLC.

  • ELISA

    • Immobilized Human R-Spondin1/RSPO1 Protein, Human (Cat. No. UA040347) at 5 μg/mL (100 μL/well) can bind LGR5 / GPR49 His Tag Protein, Cynomolgus (Cat. No. UA016188) with a linear range of 8.6-10.06 ng/mL (Routinely tested).

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