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物种
Human分子别名
Kallikrein-5, SCTE, Kallikrein-like protein 2Accession
NP_036559.1表达序列
Val23-Ser293 with His Tag at the C-Terminus
表达宿主
HEK293分子量
35-40kDa (Reducing)
纯度
＞95% by SDS-PAGE活性
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >5000pmol/min/µg .标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
50 mM MES, 150 mM NaCl, pH 5.5
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1.Palacios Serrato EG, Medina-Abreu KH, Oropeza-Martínez E, Jacinto-Alemán LF, Macías-Silva M, Tecalco-Cruz AC. ADAMTS-8 and kallikrein-related peptidases 10 and 5 proteases also have a tumor suppression role. World J Clin Oncol. 2025 Nov 24;16(11):110202.
2.Hyunjoon Kim et al.The host protease KLK5 primes and activates spike proteins to promote human betacoronavirus replication and lung inflammation.Sci. Signal.17,eadn3785(2024).
KLK5 protein (kallikrein-related peptidase 5) is a secreted serine protease encoded by the KLK5 gene, featuring a signal peptide and a catalytically active trypsin-like domain in its structure, with predominant expression in epithelial tissues such as skin and salivary glands. Physiologically, KLK5 participates in epidermal desquamation by cleaving cell adhesion molecules and activates antimicrobial peptide precursors to maintain skin barrier homeostasis, while also serving as a key host protease that cleaves coronavirus spike proteins to facilitate viral entry. Clinically, aberrant expression of KLK5 is closely associated with multiple diseases: its dysregulated activity can lead to inflammatory skin disorders including Netherton syndrome, atopic dermatitis, and rosacea; in oncology, it exhibits a complex dual role—with high expression in ovarian cancer indicating poor prognosis, whereas low expression in testicular cancer correlates with advanced disease progression. In recent years, it has also been linked to low type 2 inflammation-associated asthma, establishing KLK5 as an important therapeutic target for the development of anti-coronavirus and anti-inflammatory skin disease drugs.
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