分享产品介绍 评论(0)
物种
Human分子别名
Complement C1qAccession
P02745(C1qA), P02746(C1qB), P02747(C1qC)表达序列
C1qA :Glu23-Ala245
C1qB :Gln28-Ala253
C1qC : Asn29-Asp245 with Flag Tag at the C-Terminus表达宿主
CHO分子量
C1qA,20-35kDa(Reducing); C1qB,20-35kDa(Reducing); C1qC,20-35kDa(Reducing)纯度
＞90% by SDS-PAGE活性
Immobilized Anti-CD20 Monoclonal Antibody (Rituximab) (Cat. No. S0B0570) at 10 μg/mL (100 μL/well) can bind C1q Flag Tag Protein, Human(Cat. No. S0B0570) with EC50 of 0.60-0.74 μg/ml.标记
Unconjugated标签
Flag Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Xu Y, Zhang H, Jiao X, Zhang Y, Yin G, Wang C, Du Z, Liang M, Gao X, Gu Z, Jiang Y, Du B, Bi X. Dysregulations of C1QA, C1QB, C1QC and C5AR1 as candidate biomarkers of vascular dementia. NPJ Aging. 2025 May 25;11(1):42.
2.Son, M. Understanding the contextual functions of C1q and LAIR-1 and their applications. Exp Mol Med 54, 567–572 (2022).
Complement C1q is the recognition subunit of the classical complement pathway. This ~460 kDa hexameric protein has a unique "bouquet of tulips" structure, with six globular heads connected to a collagen-like stalk. Each head binds to ligands such as antibody Fc regions (IgG1, IgG2, IgG3, and IgM), pentraxins, and apoptotic cells. This binding activates C1r and C1s proteases, triggering the complement cascade that drives opsonization, inflammation, and pathogen lysis. Beyond immunity, C1q is essential for clearing apoptotic cells and immune complexes, maintaining self-tolerance. C1q deficiency is strongly linked to systemic lupus erythematosus (SLE), highlighting its critical role in immune homeostasis.
电泳
2μg (R: reducing condition, N:non-reducing condition).
ELISA
Immobilized Anti-CD20 Monoclonal Antibody (Rituximab) (Cat. No. S0B0570) at 10 μg/mL (100 μL/well) can bind C1q Flag Tag Protein, Human(Cat. No. S0B0570) with EC50 of 0.60-0.74 μg/ml.
评论(0)