PAPP-A His Tag Protein, Human

Pappalysin-1,PAPPA

价格￥1,920.00 （供应商现货： 3-5个工作日）

货号 UA016163

规格

25μg100μg500μg1mg

数量

产品介绍

产品介绍评论(0)

产品规格

物种

Human
分子别名

Pappalysin-1, PAPPA
Accession

Q13219
表达序列

Glu82-Gly1627 with His Tag at the C-Terminus
表达宿主

HEK293
分子量

50-55kDa&170-180kDa&＞180kDa (Reducing)
纯度

＞95% by SDS-PAGE
标记

Unconjugated
标签

His Tag
性状

Lyophilized Powder
缓冲体系

PBS, pH7.4, 5% trehalose
溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用

1.Lawrence JB, Oxvig C, Overgaard MT, Sottrup-Jensen L, Gleich GJ, Hays LG, Yates JR 3rd, Conover CA. The insulin-like growth factor (IGF)-dependent IGF binding protein-4 protease secreted by human fibroblasts is pregnancy-associated plasma protein-A. Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3149-53.

背景介绍

PAPP-A (Pregnancy-Associated Plasma Protein-A) is a zinc metalloproteinase comprising multiple functional modules including LNR1, LNR2, M1-M5 metalloprotease domains, and CPD (CUB and protease-like domain); its activity is covalently regulated by endogenous inhibitors proMBP and STC2 through steric hindrance mechanisms, and cryo-EM structural studies published in Cell Discovery and Nature Communications in 2024 first resolved the three-dimensional structures of these complexes, laying the foundation for targeted drug design; functionally, PAPP-A specifically cleaves insulin-like growth factor binding proteins (IGFBP-2, -4, -5) to release active IGF-I/II, serving as a key regulator of the IGF signaling pathway involved in fetal growth and development, bone formation, tissue repair, and metabolic regulation; clinically, PAPP-A is a core serum biomarker for first-trimester Down syndrome screening (low levels indicating fetal aneuploidy risk), with low PAPP-A also associated with fetal growth restriction, preeclampsia, and preterm delivery, while elevated levels are important biomarkers for atherosclerotic plaque instability and acute coronary syndrome, and its overexpression in ovarian cancer, breast cancer, Ewing sarcoma, and other malignancies promotes tumor progression, making it a potential target for cardiovascular risk assessment and antitumor therapy.

操作步骤

实验方法
实验原理：PAPP-A为活性蛋白酶，可在本活性检测方案规定的反应条件下切割胰岛素样生长因子结合蛋白5（IGFBP-5）。
实验材料
1.检测缓冲液: 50 mM Tris, 10 mM CaCl₂, 150 mM NaCl (TCN)
2.PAPP-A His Tag Protein, Human
3.Human IGFBP5 Protein, His Tag (S0A0185)
4.4-20% SDS-PAGE gel
5.5×reducing SDS-PAGE gel buffer
6.SDS-PAGE
实验步骤
1.用检测缓冲液将重组人PAPP-A稀释至10 μg/mL。
2.用检测缓冲液将重组人IGFBP-5稀释至200 μg/mL。
3.取等体积的10 μg/mL PAPP-A溶液与200 μg/mL IGFBP-5溶液混合；同时设置两组空白对照，对照组以检测缓冲液替代PAPP-A蛋白溶液。
4.将反应样品及其中一份对照组样品置于37 ℃条件下孵育30分钟；另一份对照组样品全程置于- 20 ℃冷藏保存。
5.从各反应管中分别取20 μL反应液，与5 μL 5×还原性SDS-PAGE上样缓冲液并混匀。
6.取20 μL混合液上样（每泳道），进行SDS-PAGE电泳；随后通过蛋白染色分析蛋白切割结果。