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物种
Cynomolgus分子别名
TSLPAccession
A0A7N9CAT7表达序列
Tyr29-Gln159(R127A, R130S) with His Tag at the C-Terminus
表达宿主
HEK293分子量
20-30kDa (Reducing)纯度
＞95% by SDS-PAGE,＞90% by HPLC活性
1. Immobilized TSLP(R127A, R130S) His Tag Protein, Cynomolgus (Cat. No.UA016148) at 1.0μg/mL (100μL/well) can bind TSLPR Fc Chimera Protein, Human(Cat. No.UA010907) with EC50 of 0.12-0.79ng/mL. 2.Immobilized TSLP(R127A, R130S) His Tag Protein, Cynomolgus (Cat. No.UA016148) at 1.0μg/mL (100μL/well) can bind Anti-TSLP Monoclonal Antibody (Tezepelumab)(Cat. No.S0B7094) with EC50 of 0.74-2.45ng/mL.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Verstraete K, Peelman F, Braun H, Lopez J, Van Rompaey D, Dansercoer A, Vandenberghe I, Pauwels K, Tavernier J, Lambrecht BN, Hammad H, De Winter H, Beyaert R, Lippens G, Savvides SN. Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma. Nat Commun. 2017 Apr 3;8:14937.
2.Tezepelumab in Adults with Uncontrolled Asthma. N Engl J Med. 2019 May 23;380(21):2082. doi: 10.1056/NEJMx180026. Epub 2019 Apr 18. Erratum for: N Engl J Med. 2017 Sep 7;377(10):936-946.
TSLP(R127A, R130S) is a full-length recombinant thymic stromal lymphopoietin stabilized by R127A and R130S double mutations, which prevent proteolytic truncation during expression by disrupting the furin cleavage site in the CD loop region (125-131 RRKRK basic cassette), thereby preserving the open four-helix bundle core (αA-αD) and dual-receptor binding activity for TSLPR/IL-7Rα; this mutant serves as a homogeneous, highly active standard antigen widely used for screening, affinity determination, and epitope mapping of anti-TSLP antibodies (e.g., Tezepelumab), as well as cross-reactivity assessment in non-human primate species such as cynomolgus monkeys, providing an indispensable molecular tool for the development of TSLP-targeted therapeutics against asthma and atopic dermatitis.
电泳
2μg (R: reducing condition, N:non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of TSLP(R127A, R130S) His Tag Protein, Cynomolgus is more than 90% determined by SEC-HPLC.
ELISA
Immobilized TSLP(R127A, R130S) His Tag Protein, Cynomolgus (Cat. No.UA016148) at 1.0μg/mL (100μL/well) can bind TSLPR Fc Chimera Protein, Human(Cat. No.UA010907) with EC50 of 0.12-0.79ng/mL.
Immobilized TSLP(R127A, R130S) His Tag Protein, Cynomolgus (Cat. No.UA016148) at 1.0μg/mL (100μL/well) can bind Anti-TSLP Monoclonal Antibody (Tezepelumab)(Cat. No.S0B7094) with EC50 of 0.74-2.45ng/mL.
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