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产品中心 药物靶点和Fc受体 药物靶点

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TSLP His Tag Protein, Cynomolgus

TSLP

价格 ￥1,290.00 （ 1-2周）
货号 UA016147
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Cynomolgus

  • 分子别名

    TSLP

  • Accession

    A0A7N9CAT7

  • 表达序列

    Tyr29-Gln159 with His Tag at the C-Terminus

  • 表达宿主

    HEK293

  • 分子量

    8-10 kDa&15-20 kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE,＞90% by HPLC

  • 活性

    1.Immobilized TSLP His Tag Protein, Cynomolgus (Cat. No.UA016147) at 1.0μg/mL (100μL/well) can bind TSLPR Fc Chimera Protein, Human(Cat. No.UA010907) with EC50 of 0.72-2.84ng/mL. 2. Immobilized TSLP His Tag Protein, Cynomolgus (Cat. No.UA016147) at 1.0μg/mL (100μL/well) can bind Anti-TSLP Monoclonal Antibody (Tezepelumab)(Cat. No.S0B7094) with EC50 of 1.29-4.69ng/mL.

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Cheng DT, Ma C, Niewoehner J, Dahl M, Tsai A, Zhang J, Gonsiorek W, Apparsundaram S, Pashine A, Ravindran P, Jung J, Hang J, Allard J, Bitter H, Tribouley C, Narula S, Wilson S, Fuentes ME. Thymic stromal lymphopoietin receptor blockade reduces allergic inflammation in a cynomolgus monkey model of asthma. J Allergy Clin Immunol. 2013 Aug;132(2):455-62.
    2.Tezepelumab in Adults with Uncontrolled Asthma. N Engl J Med. 2019 May 23;380(21):2082. doi: 10.1056/NEJMx180026. Epub 2019 Apr 18. Erratum for: N Engl J Med. 2017 Sep 7;377(10):936-946.
    3.Verstraete K, van Schie L, Vyncke L, Bloch Y, Tavernier J, Pauwels E, Peelman F, Savvides SN. Structural basis of the proinflammatory signaling complex mediated by TSLP. Nat Struct Mol Biol. 2014 Apr;21(4):375-82.

背景介绍

  • TSLP (thymic stromal lymphopoietin) is an IL-7-like cytokine produced by epithelial cells that activates the JAK/STAT signaling pathway through a ternary complex composed of its receptor TSLPR and IL-7Rα, driving dendritic cell maturation and Th2-type immune responses, and serving as a core upstream regulator of type 2 inflammation (asthma, atopic dermatitis). Due to >95% sequence homology of TSLP between cynomolgus monkeys and humans, cynomolgus monkeys have become a critical species for nonclinical safety evaluation of anti-TSLP therapeutics such as tezepelumab; their asthma models have effectively validated the in vivo efficacy of TSLP/TSLPR blockade strategies, providing irreplaceable translational medicine evidence for the development of TSLP-targeting antibody drugs.

  • 电泳

    • 2μg (R: reducing condition, N:non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • The purity of TSLP His Tag Protein, Cynomolgus is more than 90% determined by SEC-HPLC.

  • ELISA

    • Immobilized TSLP His Tag Protein, Cynomolgus (Cat. No.UA016147) at 1.0μg/mL (100μL/well) can bind TSLPR Fc Chimera Protein, Human(Cat. No.UA010907) with EC50 of 0.72-2.84ng/mL.

    • Immobilized TSLP His Tag Protein, Cynomolgus (Cat. No.UA016147) at 1.0μg/mL (100μL/well) can bind Anti-TSLP Monoclonal Antibody (Tezepelumab)(Cat. No.S0B7094) with EC50 of 1.29-4.69ng/mL.

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