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物种
Human分子别名
MMP8, Matrix metalloproteinase-8, PMNL-CL, CLG1Accession
P22894表达序列
Phe21-Gly467 with His Tag at C-Terminus
表达宿主
HEK293分子量
60-75kDa (Reducing)
纯度
> 95% by SDS-PAGE,90% by HPLC内毒素含量
<0.1EU/μg活性
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001). The specific activity is >700 pmol/min/µg, as measured under the described conditions.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Cathomas, F., Lin, HY., Chan, K.L. et al. Circulating myeloid-derived MMP8 in stress susceptibility and depression. Nature 626, 1108–1115 (2024).
2.Aji NRAS, Yucel-Lindberg T, Räisänen IT, Kuula H, Nieminen MT, Mc Crudden MTC, Listyarifah D, Lundmark A, Lundy FT, Gupta S, Sorsa T. In Vivo Regulation of Active Matrix Metalloproteinase-8 (aMMP-8) in Periodontitis: From Transcriptomics to Real-Time Online Diagnostics and Treatment Monitoring. Diagnostics (Basel). 2024 May 15;14(10):1011.
MMP-8, also known as matrix metalloproteinase-8 or neutrophil collagenase, is primarily synthesized by neutrophils and stored within their specific granules, from which it can be rapidly released upon inflammatory stimulation. This protein belongs to the zinc-dependent endopeptidase family and exhibits a highly conserved structure comprising a signal peptide, a pro-peptide (containing the "cysteine switch" that maintains zymogen stability), a catalytic domain harboring the zinc-binding motif (HEXXHXXGXXH), and a hemopexin-like domain involved in substrate recognition; upon extracellular cleavage of the pro-peptide by proteases such as plasmin, the zymogen is converted into its active form (aMMP-8) with potent collagenolytic activity. Functionally, aMMP-8 primarily acts to specifically cleave type I, II, and III collagens, thereby participating in tissue remodeling and repair under physiological conditions, whereas under pathological states it serves as a key effector molecule mediating inflammatory tissue destruction in periodontitis, arthritis, and other inflammatory diseases; recent studies have further revealed that circulating myeloid-derived MMP-8 can traverse the blood–brain barrier to modulate neuroinflammation, contributing to the pathogenesis of psychiatric disorders such as depression, and has garnered growing attention as a novel target for chronic inflammatory pain. Clinically, salivary or gingival crevicular fluid levels of aMMP-8 have been established as one of the most reliable biomarkers for the early diagnosis, disease activity assessment, and therapeutic monitoring of periodontitis, with chairside point-of-care testing technologies developed on this basis now being maturely applied in clinical practice; concurrently, its roles as a risk predictor for periodontitis complicated by obesity and for periodontal complications following radiotherapy in head and neck cancer patients, as well as a potential therapeutic target for pain management and neuropsychiatric interventions, are propelling its translational application from stomatology toward precision diagnostics and therapeutics in systemic diseases
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of MMP-9 His Tag Protein, Mouse is more than 90 % as determined by SEC-HPLC.
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