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物种
Human分子别名
CD5, LEU1, T1, Tp67Accession
P06127表达序列
Leu 159 - Pro 372 with hIgG1 Fc Tag at the C-Terminus
表达宿主
HEK293分子量
55-70kDa (Reducing)
纯度
＞95% by SDS-PAGE & HPLC活性
Immobilized CD5(159-372) Fc Chimera Protein, Human (Cat. No. UA016083) at 1.0μg/mL (100μL/well) can bind CD5 Recombinant Rabbit mAb (SDT-2857-16) with EC50 of 2.78-5.40 ng/mL.标记
Unconjugated标签
Human IgG1 Fc性状
Lyophilized Powder缓冲体系
PBS, pH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Van de Velde, H., et al. (1991). The B-cell surface protein CD72/Lyb-2 is the ligand for CD5. Nature, 351(6328), 662-665.
2. McAlister, M.S., et al. (1998). Structural analysis of the CD5 antigen--expression, disulphide bond analysis and physical characterisation of CD5 scavenger receptor superfamily domain 1.
3. Garza-Garcia, A., et al. (2008). Three-dimensional solution structure and conformational plasticity of the N-terminal scavenger receptor cysteine-rich domain of human CD5. Journal of Molecular Biology, 378(1), 129-144.
CD5 is a type I transmembrane glycoprotein with a molecular weight of approximately 67 kDa. Its extracellular region is composed of three group B scavenger receptor cysteine-rich domains (D1, D2, D3), each containing 8 cysteines. The cytoplasmic domain comprises 93 amino acids and includes an immunoreceptor tyrosine-based inhibitory motif-like domain responsible for recruiting the protein tyrosine phosphatase SHP-1.
The 159-372 amino acid fragment of the human CD5 protein corresponds to the second and third SRCR domains of its extracellular region. This fragment is typically located in the membrane-proximal region of full-length CD5. By lacking the N-terminal first SRCR domain, its potential conformation and ligand-binding properties may differ from the full extracellular region, making it an important target for functional studies.
This fragment contains multiple functional sites and may be involved in interactions with other immune molecules, such as CD72 on the surface of B cells. Due to the high conservation of SRCR domains, this fragment likely plays a crucial role in maintaining the structural integrity of the whole protein.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of CD5(159-372) Fc Chimera Protein, Human is more than 95% determined by SEC-HPLC.
反相高效液相色谱(RP-HPLC)
The purity of CD5(159-372) Fc Chimera Protein, Human is more than 95% determined by RP-HPLC.
ELISA
Immobilized CD5(159-372) Fc Chimera Protein, Human (Cat. No. UA016083) at 1.0μg/mL (100μL/well) can bind CD5 Recombinant Rabbit mAb (SDT-2857-16) (Cat. No. S0B2441) with EC50 of 2.54-5.96 ng/mL.
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