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物种
Human分子别名
ErbB3, Her3, FERLK, LCCS2, VSCN1Accession
P21860表达序列
Ser20-Thr643 with His Tag at C-terminal
表达宿主
HEK293分子量
100-130kDa (Reducing)纯度
＞95% by SDS-PAGE & HPLC活性
1. Immobilized ErbB3 / Her3 His Tag Protein, Human (Cat. No.UA016070) at 2.0μg/mL (100μL/well) can bind Anti-Human HER3 Monoclonal Antibody (Patritumab) with EC50 of 0.08-0.16ng/mL. 2. Immobilized ErbB3 / Her3 His Tag Protein, Human (Cat. No. UA016070) at 2.0μg/mL (100μL/well) can bind Human NRG1 Beta 1 Protein, Fc Tag, premium grade with EC50 of 1.90-2.40 ng/mL.标记
Unconjugated性状
Lyophilized Powder缓冲体系
PBS, pH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Ma, J., et al. (2026). HER3 beyond the canonical paradigm: a versatile signaling hub in oncogenesis and therapeutic resistance. Journal of Translational Medicine, 24(1), 123.
2. Wang, Y., & Zhang, X. (2024). HER3: Updates and current biology function, targeted therapy and pathologic detecting methods. Life Sciences, 357, 123087.
3. Kiavue, N., et al. (2020). ERBB3 mutations in cancer: biological aspects, prevalence and therapeutics. Oncogene, 39(3), 487-502.
Human Epidermal Growth Factor Receptor 3 (HER3/ErbB3) is a distinctive member of the epidermal growth factor receptor (EGFR/ErbB) family, which plays a central role in cell proliferation, differentiation, migration, and survival. Its most prominent feature is that its intracellular kinase domain exhibits extremely low, if not absent, catalytic activity compared to other family members (EGFR, HER2, HER4). Consequently, HER3 cannot signal alone and must heterodimerize with other active family members (primarily HER2) to be activated and initiate downstream signaling pathways.
Structurally, HER3, like other ErbB family members, consists of an extracellular ligand-binding domain, a single transmembrane helix, and an intracellular domain containing the kinase domain. Its extracellular domain specifically binds to ligands such as Neuregulin (NRG), triggering a conformational change that exposes its dimerization arm, enabling it to bind to EGFR or HER2.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of ErbB3/Her3 His Tag Protein, Human is more than 95% determined by SEC-HPLC.
ELISA
Immobilized ErbB3 / Her3 His Tag Protein, Human (Cat. No.UA016070) at 2.0μg/mL (100μL/well) can bind Anti-Human HER3 Monoclonal Antibody (Patritumab) with EC50 of 0.08-0.16ng/mL.
Immobilized ErbB3 / Her3 His Tag Protein, Human (Cat. No. UA016070) at 2.0μg/mL (100μL/well) can bind Human NRG1 Beta 1 Protein, Fc Tag, premium grade with EC50 of 1.90-2.40 ng/mL.
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