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产品中心 药物靶点和Fc受体 药物靶点

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BID His Tag Protein, Human

BH3 interacting domain death agonist

价格 ￥1,290.00 （ 供应商现货 ： 3-5个工作日）
货号 UA016067
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    BH3 interacting domain death agonist

  • Accession

    P55957

  • 表达序列

    Met1-Asp195 with His Tag at the C-Terminus

  • 表达宿主

    E.coli

  • 分子量

    15-25kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 活性

    1.Immobilized BID His Tag Protein, Human (Cat. No. UA016067) at 10.0μg/mL (100μL/well) can bind Biotinylated Bcl-x/BCL2L1 His Tag Protein, Human (Cat. No. UA011326) with EC50 of 3.51-4.23 ng/mL. 2.Immobilized BID His Tag Protein, Human (Cat. No. UA016067) at 10.0μg/mL (100μL/well) can bind Biotinylated Bcl-x/BCL2L1 Protein, Human (Cat. No. UA011327) with EC50 of 4.56-7.53 ng/mL.

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Wang K, Yin XM, Chao DT, Milliman CL, Korsmeyer SJ. BID: a novel BH3 domain-only death agonist. Genes Dev. 1996 Nov 15;10(22):2859-69.
    2.Li H, Zhu H, Xu CJ, Yuan J. Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell. 1998 Aug 21;94(4):491-501.

背景介绍

  • BID (BH3 Interacting Domain Death Agonist) is a critical BH3-only pro-apoptotic protein containing an unstructured N-terminal domain and a conserved C-terminal BH3 domain. Upon cleavage by caspase-8, BID generates the active fragment tBID, which subsequently activates Bax/Bak and induces mitochondrial outer membrane permeabilization. Serving as an essential bridge between the death receptor and mitochondrial pathways, BID not only mediates apoptosis but also participates in hematopoietic homeostasis, tumor suppression, and mitochondrial structural maintenance. Aberrant BID expression is closely associated with various diseases: in tumors, its downregulation inhibits apoptosis and promotes drug resistance; during SARS-CoV-2 infection, the virus modulates BID to balance host cell survival and death; moreover, abnormal expression of BID alternative splicing isoforms is significantly correlated with the progression and poor prognosis of malignancies such as colorectal cancer, establishing BID as a potential diagnostic biomarker and therapeutic target.

  • 电泳

    • 2μg (R: reducing condition, N:non-reducing condition).

  • ELISA

    • Immobilized BID His Tag Protein, Human (Cat. No. UA016067) at 10.0μg/mL (100μL/well) can bind Biotinylated Bcl-x/BCL2L1 His Tag Protein, Human (Cat. No. UA011326) with EC50 of 3.51-4.23 ng/mL.

    • Immobilized BID His Tag Protein, Human (Cat. No. UA016067) at 10.0μg/mL (100μL/well) can bind Biotinylated Bcl-x/BCL2L1 Protein, Human (Cat. No. UA011327) with EC50 of 4.56-7.53 ng/mL.

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