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物种
Cynomolgus分子别名
Interleukin-18-binding proteinAccession
A0A2K5UDJ4表达序列
Thr28-Pro207 with His Tag at the C-Terminus
表达宿主
HEK293分子量
35-70kDa (Reducing)
纯度
＞95% by SDS-PAGE活性
Immobilized IL-18 Protein, Human (Cat. No. UA040111) at 1.0μg/mL (100μL/well) can bind IL-18BP His Tag Protein, Cynomolgus (Cat. No. UA016066) with EC50 of 4.36-7.07 ng/mL.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Novick D. IL-18 and IL-18BP: A Unique Dyad in Health and Disease. Int J Mol Sci. 2024 Dec 17;25(24):13505.
2.Landy E, Carol H, Ring A, Canna S. Biological and clinical roles of IL-18 in inflammatory diseases. Nat Rev Rheumatol. 2024 Jan;20(1):33-47.
3.Wang X, Wang L, Wen X, Zhang L, Jiang X, He G. Interleukin-18 and IL-18BP in inflammatory dermatological diseases. Front Immunol. 2023 Jan 18;14:955369.
IL-18BP (Interleukin-18 Binding Protein) is a secreted immunoregulatory glycoprotein belonging to the IL-1 receptor family, whose structure contains a single Ig-like domain that binds IL-18 with high affinity (Kd≈400 pM) and blocks its interaction with the IL-18Rα/β receptors, thereby inhibiting NF-κB and AP-1 signaling pathways and downstream IFN-γ production; recombinant IL-18BP His-tagged protein derived from Cynomolgus monkeys holds critical clinical significance in drug development, serving as a natural antagonist for treating NLRC4-related autoinflammatory diseases, rheumatoid arthritis, and other Th1-mediated autoimmune disorders, while its identification as a secreted immune checkpoint has revealed the resistance mechanism of IL-18 immunotherapy, driving the development of next-generation biologics including anti-IL-18BP antibodies and IL-18 mutants, with this protein also serving as a biomarker for radiation injury and a potential target for radioprotective agents.
生物活性
Measured by its ability to inhibit the IL-18-induced response of KG-1 human acute myelogenous leukemia cells. The IC50 for this effect is 12-21 ng/mL in the presence of 20 ng/mL of recombinant human IL-18.
电泳
2μg (R: reducing condition, N:non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of IL-18BP His Tag Protein, Cynomolgus is more than 90% determined by SEC-HPLC.
ELISA
Immobilized IL-18 Protein, Human (Cat. No. UA040111) at 1.0μg/mL (100μL/well) can bind IL-18BP His Tag Protein, Cynomolgus (Cat. No. UA016066) with EC50 of 4.36-7.07 ng/mL.
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