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物种
Human分子别名
IBD17,IL-23 receptorAccession
Q5VWK5-1表达序列
Gly24-Gly355 with hIgG1 Fc at the C-Terminus
表达宿主
HEK293分子量
90-110kDa (Reducing)
纯度
＞95% by SDS-PAGE & HPLC活性
1.Immobilized Human IL-23 alpha & IL-12 beta Heterodimer Protein, No tag&His tag (Cat. No. S0A4041) at 1.0μg/mL (100μL/well) can bind IL-23 R Fc Chimera Protein, Human (Cat. No. UA016005) with EC50 of 20.7-29.4ng/mL. 2.IL-23 R Fc Chimera Protein, Human (Cat. No. UA016005) captured on Protein A Chip can bind Human IL-23 alpha & IL-12 beta Heterodimer Protein, No tag&His tag(Cat. No. S0A4041) with an affinity constant of 17.56 nM as determined in a SPR assay .标记
Unconjugated标签
Human IgG1 Fc性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Benson JM, Sachs CW, Treacy G, Zhou H, Pendley CE, Brodmerkel CM, Shankar G, Mascelli MA. Therapeutic targeting of the IL-12/23 pathways: generation and characterization of ustekinumab. Nat Biotechnol. 2011 Jul;29(7):615-24.
2.Hue S, Ahern P, Buonocore S, Kullberg MC, Cua DJ, McKenzie BS, Powrie F, Maloy KJ. Interleukin-23 drives innate and T cell-mediated intestinal inflammation. J Exp Med. 2006 Oct 30;203(11):2473-83.
- Interleukin-23 (IL-23) is a heterodimeric cytokine composed of p19 and p40 subunits. Its functional receptor, IL-23R, combines with the IL-12Rβ1 subunit to form a type I cytokine receptor complex. This complex activates the downstream JAK2-STAT3 signaling pathway, regulating the proliferation, differentiation, and secretion of inflammatory factors in immune cells such as T cells and NK cells. It plays a central role in sustaining Th17 cell-mediated inflammatory responses. Clinically, abnormal activation of the IL-23/IL-23R pathway is a key pathogenic mechanism in autoimmune diseases such as psoriasis and inflammatory bowel disease. Additionally, it promotes tumorigenesis within the tumor microenvironment, making it a critical therapeutic target.
电泳
2μg (R: reducing condition, N:non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of IL-23 R Fc Chimera Protein, Human is more than 95% determined by SEC-HPLC.
ELISA
Immobilized Human IL-23 alpha & IL-12 beta Heterodimer Protein, No tag&His tag (Cat. No. S0A4041) at 1.0μg/mL (100μL/well) can bind IL-23 R Fc Chimera Protein, Human (Cat. No. UA016005) with EC50 of 20.7-29.4ng/mL.
SPR
IL-23 R Fc Chimera Protein, Human (Cat. No. UA016005) captured on Protein A Chip can bind Human IL-23 alpha & IL-12 beta Heterodimer Protein, No tag&His tag(Cat. No. S0A4041) with an affinity constant of 17.56 nM as determined in a SPR assay .
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