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物种
Mouse分子别名
GHBP,GH receptorAccession
Q3UP14表达序列
Thr25-Gln273 with hIgG1 Fc Tag at the C-Terminus
表达宿主
HEK293分子量
50-75kDa (Reducing)纯度
＞95% by SDS-PAGE & > 90% by HPLC标记
Unconjugated标签
Human IgG1 Fc性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Le Roith D, Bondy C, Yakar S, Liu JL, Butler A. The somatomedin hypothesis: 2001. Endocr Rev. 2001 Feb;22(1):53-74.
2.Kermani H, Goffinet L, Mottet M, Bodart G, Morrhaye G, Dardenne O, Renard C, Overbergh L, Baron F, Beguin Y, Geenen V, Martens HJ. Expression of the growth hormone/insulin-like growth factor axis during Balb/c thymus ontogeny and effects of growth hormone upon ex vivo T cell differentiation. Neuroimmunomodulation.
Growth hormone receptor (GHR), also known as growth hormone-binding protein (GHBP), is a single-pass type I transmembrane protein belonging to the cytokine receptor family. Its structure contains a typical fibronectin type-III domain and is predominantly expressed in the liver and skeletal muscle, while various isoforms are distributed in multiple tissues such as the kidneys and placenta. Upon binding to pituitary-derived growth hormone, this receptor activates the JAK2/STAT5 signaling pathway to regulate body growth and metabolism. The soluble GHBP, formed through proteolytic cleavage of the extracellular domain, serves as a circulating reservoir for growth hormone and negatively modulates signal transduction. Clinically, GHR gene defects are directly linked to multiple growth disorders, including severe growth hormone resistance such as Laron syndrome—characterized by growth retardation and impaired insulin-like growth factor I production—as well as idiopathic short stature and other inherited growth abnormalities. Thus, GHR is not only a key regulator of growth but also an important diagnostic and therapeutic target for related endocrine disorders.
电泳
2μg (R: reducing condition, N:non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of GHR Fc Chimera Protein, Mouse is more than 90% determined by SEC-HPLC.
ELISA
Immobilized Growth hormone Protein, Human (Cat. No. UA040020) at 2.0μg/mL (100μL/well) can bind GHR Fc Chimera Protein, Mouse (Cat. No. UA011366) with EC50 of 1.89-3.04 ng/mL.
Immobilized Human GH, His tag (Cat. No.S0A8004) at 2.0μg/mL (100μL/well) can bind GHR Fc Chimera Protein, Mouse (Cat. No. UA011366) with EC50 of 1.44-2.12 ng/mL.
Immobilized Growth hormone Protein, Mouse (Cat. No. UA040491) at 2.0μg/mL (100μL/well) can bind GHR Fc Chimera Protein, Mouse (Cat. No. UA011366) with EC50 of 2.16-3.80 ng/mL.
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