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产品中心 药物靶点和Fc受体 药物靶点

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BAFF&APRIL Heterotrimer His Tag Protein, Human

BAFF&APRIL

价格 ￥1,400.00 （ 供应商现货 ： 3-5个工作日）
货号 UA011338
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    BAFF&APRIL

  • Accession

    Q9Y275, O75888

  • 表达序列

    Glu140-Leu285&Gln111-Leu250 with His Tag at the N-Terminus

  • 表达宿主

    HEK293

  • 分子量

    45-65kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE & > 95% SEC-HPLC

  • 活性

    1.Immobilized BAFF&APRIL Heterotrimer His Tag Protein, Human (Cat. No. UA011338) at 2.0μg/mL (100μL/well) can bind Biotinylated BAFFR/TNFRSF13C Fc&Avi Tag Protein, Human (Cat. No. UA011346) with EC50 of 24.45-41.71 ng/mL. 2.Immobilized BAFF&APRIL Heterotrimer His Tag Protein, Human (Cat. No. UA011338) at 2.0μg/mL (100μL/well) can bind TACI/CD267/TNFRSF13B Fc Chimera Protein, Human (Cat. No. UA011118) with EC50 of 1.65-2.67 ng/mL.

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, PH7.4, 5% trehalose

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1.Wiggins CD, Sangés Ametllé M, Wu C, Boswell KL, Watkins TA, Koup RA, Lauffenburger DA. Multivariate analysis of glycogenes reveals coordinated regulation of immunoglobulin glycosylation in an immortalized human B cell system. bioRxiv [Preprint]. 2025 Oct 2:2025.09.30.679651.
    2.Rybka G, Dziedzic R, Węglarczyk K, Milewski M, Siwiec-Koźlik A, Dziedzina S, Sanak M, Musiał J, Siedlar M, Korkosz M, Kosałka-Węgiel J. Serum levels of APRIL, BAFF, and IL-10 in systemic lupus erythematosus have limited utility as biomarkers for disease activity or flare prediction. Clin Rheumatol. 2025 Nov 16.

背景介绍

  • BAFF (B-cell activating factor) and APRIL (a proliferation-inducing ligand) are members of the tumor necrosis factor (TNF) superfamily. They typically exist as homotrimers but can also form heterotrimers. Secreted by activated myeloid cells, they exert immunomodulatory effects by regulating B-cell differentiation, proliferation, survival, and effector functions. Clinically, dysregulated expression of the BAFF/APRIL system is closely associated with autoimmune diseases, particularly with significantly elevated levels observed in patients with systemic lupus erythematosus (SLE). The detection of circulating heterotrimers in the serum of patients with systemic immune rheumatic diseases suggests that these heterotrimers may represent a novel therapeutic target for rheumatic and autoimmune disorders.

  • 电泳

    • 1μg (R: reducing condition, N:non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • The purity of BAFF&APRIL Heterotrimer His Tag Protein, Human is more than 95% determined by SEC-HPLC.

  • ELISA

    • Immobilized BAFF&APRIL Heterotrimer His Tag Protein, Human (Cat. No. UA011338) at 2.0μg/mL (100μL/well) can bind Biotinylated BAFFR/TNFRSF13C Fc&Avi Tag Protein, Human (Cat. No. UA011346) with EC50 of 24.45-41.71 ng/mL.

    • Immobilized BAFF&APRIL Heterotrimer His Tag Protein, Human (Cat. No. UA011338) at 2.0μg/mL (100μL/well) can bind TACI/CD267/TNFRSF13B Fc Chimera Protein, Human (Cat. No. UA011118) with EC50 of 1.65-2.67 ng/mL.

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