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物种
Human分子别名
CD318, TRASK, UNQ2486, Membrane glycoprotein gp140Accession
Q9H5V8-1表达序列
Phe30-Thr667 with His Tag at the C-Terminus
表达宿主
HEK293分子量
100-120kDa (Reducing)纯度
＞95% by SDS-PAGE & HPLC活性
Immobilized CDCP1 His Tag Protein, Human (Cat. No.UA011335) at 1.0μg/mL (100μL/well) can bind CDCP1 Recombinant Monoclonal Antibody with EC50 of 1.22-1.47 ng/mL.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Chiu KL, Lin YS, Kuo TT, Lo CC, Huang YK, Chang HF, Chuang EY, Lin CC, Cheng WC, Liu YN, Lai LC, Sher YP. ADAM9 enhances CDCP1 by inhibiting miR-1 through EGFR signaling activation in lung cancer metastasis. Oncotarget. 2017 Jul 18;8(29):47365-47378.
2.Zhang L, Wu Y, Li S, Guo M, Zhao J, Cao C, Zhang Y, Xiao H. Proteomic Analysis of Extracellular Vesicles Identifies CDCP1 as Critical Metastasis-Related Glycoprotein in Lung Cancer. J Extracell Vesicles. 2025 Jul;14(7):e70128.
CDCP1 (CUB Domain Containing Protein 1, also known as SIMA135 or TRASK) is a single-pass transmembrane glycoprotein featuring three extracellular CUB domains and proteolytic cleavage sites; its intracellular segment activates Src family kinases (SFKs) via Y734 phosphorylation, thereby regulating cell adhesion, motility, and anoikis resistance. This protein can bind to integrin β1 to activate FAK/PI3K/Akt signaling and functions as a hypoxia-inducible factor-2α (HIF-2α) target gene under hypoxic regulation; in breast cancer and renal cell carcinoma, CDCP1 drives metastasis through promoting fatty acid oxidation and hypoxic adaptation, respectively. Clinical studies demonstrate that CDCP1 is highly expressed in melanoma, pancreatic cancer, prostate cancer, and other solid tumors, correlating with poor patient prognosis and castration-resistant prostate cancer progression, establishing it as a potential prognostic biomarker and therapeutic target, with anti-CDCP1 antibodies (such as 4A06) currently being investigated for theranostic targeted therapy applications, offering novel precision intervention strategies for metastatic malignancies.
电泳
1μg (R: reducing condition, N:non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of CDCP1 His Tag Protein, Human is more than 95% determined by SEC-HPLC.
ELISA
Immobilized CDCP1 His Tag Protein, Human (Cat. No.UA011335) at 1.0μg/mL (100μL/well) can bind CDCP1 Recombinant Monoclonal Antibody with EC50 of 1.22-1.47 ng/mL.
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