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物种
Human分子别名
Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2L, BCLXAccession
Q07817表达序列
Met1-Arg212 with His Tag at the C-Terminus
表达宿主
E.coli分子量
25-30kDa (Reducing)纯度
＞90% by SDS-PAGE活性
Immobilized BID His Tag Protein, Human (Cat. No. UA016067) at 10.0μg/mL (100μL/well) can bind Biotinylated Bcl-x/BCL2L1 His Tag Protein, Human (Cat. No. UA011326) with EC50 of 3.51-4.23 ng/mL.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, PH7.4, 5% trehalose
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Boise LH, González-García M, Postema CE, Ding L, Lindsten T, Turka LA, Mao X, Nuñez G, Thompson CB. bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death. Cell. 1993 Aug 27;74(4):597-608.
2.Muchmore SW, Sattler M, Liang H, Meadows RP, Harlan JE, Yoon HS, Nettesheim D, Chang BS, Thompson CB, Wong SL, Ng SL, Fesik SW. X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature. 1996 May 23;381(6580):335-41.
3.Tse C, Shoemaker AR, Adickes J, Anderson MG, Chen J, Jin S, Johnson EF, Marsh KC, Mitten MJ, Nimmer P, Roberts L, Tahir SK, Xiao Y, Yang X, Zhang H, Fesik S, Rosenberg SH, Elmore SW. ABT-263: a potent and orally bioavailable Bcl-2 family inhibitor. Cancer Res. 2008 May 1;68(9):3421-8.
Bcl-x (BCL2L1) is an important anti-apoptotic protein in the Bcl-2 family, featuring two major isoforms: Bcl-xL (long form, anti-apoptotic) and Bcl-xS (short form, pro-apoptotic). Bcl-xL contains BH1-4 domains and a C-terminal transmembrane region, and inhibits mitochondrial outer membrane permeabilization by sequestering Bax/Bak or BH3-only proteins (such as Bim and Bad). Beyond regulating apoptosis, Bcl-xL suppresses autophagy through binding to Beclin-1 and participates in mitochondrial homeostasis maintenance. Bcl-xL is highly expressed in various solid tumors and is associated with chemotherapy resistance and poor prognosis; although BH3 mimetics targeting Bcl-xL (such as Navitoclax) demonstrate anti-tumor activity, their clinical application is limited by dose-limiting thrombocytopenia, as Bcl-xL is essential for platelet survival. Current research is shifting toward selective Bcl-xL inhibitors or PROTAC degradation strategies to overcome this toxicity.
电泳
1μg (R: reducing condition, N:non-reducing condition).
ELISA
Immobilized BID His Tag Protein, Human (Cat. No. UA016067) at 10.0μg/mL (100μL/well) can bind Biotinylated Bcl-x/BCL2L1 His Tag Protein, Human (Cat. No. UA011326) with EC50 of 3.51-4.23 ng/mL.
引用文献(1)
- 野马追内酯B通过p38 MAPK调控阿霉素诱导心肌病中心肌细胞凋亡及炎症的研究
陈徐波,黄春艳,胡益冰,许竞,潘轶斌
The Chinese Journal of Clinical Pharmacology. 2026 Jun 11 .
影响因子： 3.1
货号：UA011326产品名称：Bcl-x/BCL2L1 His Tag Protein, Human
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